| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
2 Department of Chemistry, University of California, Berkeley, California 94720-5230, USA
Reprint requests to: Sung-Hou Kim, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; e-mail: SHKim{at}cchem.berkeley.edu; fax: (510) 486-5272.
We have determined the crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima, TM0651 (gi 4981173), at 2.2 Å resolution by selenomethionine single-wavelength anomalous diffraction (SAD) techniques. TM0651 is a member of the haloacid dehalogenase (HAD) superfamily, with sequence homology to trehalose-6-phosphate phosphatase and sucrose-6F-phosphate phosphohydrolase. Selenomethionine labeled TM0651 crystallized in space group C2 with three monomers per asymmetric unit. Each monomer has approximate dimensions of 65 x 40 x 35 Å3, and contains two domains: a domain of known hydrolase fold characteristic of the HAD family, and a domain with a new tertiary fold consisting of a six-stranded ß-sheet surrounded by four 
-helices. There is one disulfide bond between residues Cys35 and Cys265 in each monomer. One magnesium ion and one sulfate ion are bound in the active site. The superposition of active site residues with other HAD family members indicates that TM0651 is very likely a phosphatase that acts through the formation of a phosphoaspartate intermediate, which is supported by both NMR titration data and a biochemical assay. Structural and functional database searches and the presence of many aromatic residues in the interface of the two domains suggest the substrate of TM0651 is a carbohydrate molecule. From the crystal structure and NMR data, the protein likely undergoes a conformational change upon substrate binding.
Keywords: X-ray crystallography; structural proteomics; phosphatase; HAD family; new fold; gi 4981173
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. F. Gherardini and M. Helmer-Citterich Structure-based function prediction: approaches and applications Brief Funct Genomic Proteomic, July 3, 2008; (2008) eln030v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Rangarajan, A. Proteau, J. Wagner, M.-N. Hung, A. Matte, and M. Cygler Structural Snapshots of Escherichia coli Histidinol Phosphate Phosphatase along the Reaction Pathway J. Biol. Chem., December 8, 2006; 281(49): 37930 - 37941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Rao, D. Kumaran, J. Seetharaman, J. B. Bonanno, S. K. Burley, and S. Swaminathan Crystal structure of trehalose-6-phosphate phosphatase-related protein: Biochemical and biological implications. Protein Sci., July 1, 2006; 15(7): 1735 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fieulaine, J. E. Lunn, F. Borel, and J.-L. Ferrer The Structure of a Cyanobacterial Sucrose-Phosphatase Reveals the Sugar Tongs That Release Free Sucrose in the Cell PLANT CELL, July 1, 2005; 17(7): 2049 - 2058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Shin, Y. Lou, J. Jancarik, H. Yokota, R. Kim, and S.-H. Kim Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain PNAS, September 7, 2004; 101(36): 13198 - 13203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Shi and G. C. Ferreira Probing the Active Site Loop Motif of Murine Ferrochelatase by Random Mutagenesis J. Biol. Chem., May 7, 2004; 279(19): 19977 - 19986. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
