Protein Science (2004), 13:2588-2599.
Published by Cold Spring Harbor Laboratory Press. Copyright © 2004 The Protein Society
Comparison of TFII-I gene family members deleted in Williams-Beuren syndrome
Timothy A. Hinsley1,2,
Pamela Cunliffe1,
Hannah J. Tipney1,
Andrew Brass2,3 and
May Tassabehji1
1 Academic Department of Medical Genetics, St. Mary’s Hospital, Manchester M13 OJH, United Kingdom
2 School of Biological Science and
3 Department of Computer Science, University of Manchester, Manchester M13 9PL United Kingdom
(RECEIVED April 2, 2004;
FINAL REVISION June 30, 2004;
ACCEPTED July 2, 2004)
Williams-Beuren syndrome (WBS) is a neurological disorder resulting from a microdeletion, typically 1.5 megabases in size, at 7q11.23. Atypical patients implicate genes at the telomeric end of this multigene deletion as the main candidates for the pathology of WBS in particular the unequal cognitive profile associated with the condition. We recently identified a gene (GTF2IRD2) that shares homology with other members of a unique family of transcription factors (TFII-I family), which reside in the critical telomeric region. Using bioinformatics tools this study focuses on the detailed assessment of this gene family, concentrating on their characteristic structural components such as the leucine zipper (LZ) and I-repeat elements, in an attempt to identify features that could aid functional predictions. Phylogenetic analysis identified distinct I-repeat clades shared between family members. Linking functional data to one such clade has implicated them in DNA binding. The identification of PEST, synergy control motifs, and sumoylation sites common to all family members suggest a shared mechanism regulating the stability and transcriptional activity of these factors. In addition, the identification/isolation of short truncated isoforms for each TFII-I family member implies a mode of self-regulation. The exceptionally high identity shared between GTF2I and GTF2IRD2, suggests that heterodimers as well as homodimers are possible, and indicates overlapping functions between their respective short isoforms. Such cross-reactivity between GTF2I and GTF2IRD2 short isoforms might have been the evolutionary driving force for the 7q11.23 chromosomal rearrangement not present in the syntenic region in mice.
Keywords: Williams-Beuren Syndrome; GTF2I; GTF2IRD1; GTF2IRD2; short isoforms; PEST sequence; sumoylation sites; synergy control motif
Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.04747604.
Reprint requests to: May Tassabehji, Academic Department of Medical Genetics, St. Mary’s Hospital, Hathersage Road, Manchester, M13 0JH, UK; e-mail: M.Tassabehji{at}man.ac.uk; fax: +44-(0)161-276-6606.
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