HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: ps.04781504v1 13/10/2613    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sale, K. Articles by Young, M. M. Search for Related Content PubMed PubMed Citation Articles by Sale, K. Articles by Young, M. M. Social Bookmarking                   What's this?

Optimal bundling of transmembrane helices using sparse distance constraints

Sandia National Laboratories, Livermore, California 94551-0969, USA

(RECEIVED April 16, 2004; FINAL REVISION June 23, 2004; ACCEPTED June 23, 2004)

We present a two-step approach to modeling the transmembrane spanning helical bundles of integral membrane proteins using only sparse distance constraints, such as those derived from chemical cross-linking, dipolar EPR and FRET experiments. In Step 1, using an algorithm, we developed, the conformational space of membrane protein folds matching a set of distance constraints is explored to provide initial structures for local conformational searches. In Step 2, these structures refined against a custom penalty function that incorporates both measures derived from statistical analysis of solved membrane protein structures and distance constraints obtained from experiments. We begin by describing the statistical analysis of the solved membrane protein structures from which the theoretical portion of the penalty function was derived. We then describe the penalty function, and, using a set of six test cases, demonstrate that it is capable of distinguishing helical bundles that are close to the native bundle from those that are far from the native bundle. Finally, using a set of only 27 distance constraints extracted from the literature, we show that our method successfully recovers the structure of dark-adapted rhodopsin to within 3.2 Å of the crystal structure.

Keywords: helix packing; transmembrane helices; distance constraints; molecular refinement

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.04781504.

Reprint requests to: Ken Sale, Biosystems Research Department, Sandia National Laboratories, P.O. Box 969, MS 9951, Livermore CA 94551-0969, USA; e-mail: klsale{at}sandia.gov; fax: (925) 294-3020.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. Pabuwal and Z. Li Network pattern of residue packing in helical membrane proteins and its application in membrane protein structure prediction Protein Eng. Des. Sel., January 3, 2008; (2008) gzm059v1. [Abstract] [Full Text] [PDF]

				J. A. Kovacs, M. Yeager, and R. Abagyan Computational Prediction of Atomic Structures of Helical Membrane Proteins Aided by EM Maps Biophys. J., September 15, 2007; 93(6): 1950 - 1959. [Abstract] [Full Text] [PDF]