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Protein Science (2004), 13:854-861. Published by Cold Spring Harbor Laboratory Press. Copyright © 2004 The Protein Society
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Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases

Matthias Bochtler, Sergey G. Odintsov, Malgorzata Marcyjaniak and Izabela Sabala

International Institute of Molecular and Cell Biology (IIMCB), 02-109 Warsaw, Poland Max-Planck-Institute for Molecular Cell Biology and Genetics, 01309 Dresden, Germany

(RECEIVED November 17, 2003; FINAL REVISION January 11, 2004; ACCEPTED January 13, 2004)



Abstract

Specific peptidases exist for nearly every amide linkage in peptidoglycan. In several cases, families of peptidoglycan hydrolases with different specificities turned out to be related. Here we show that lysostaphin-type peptidases and D-Ala-D-Ala metallopeptidases have similar active sites and share a core folding motif in otherwise highly divergent folds. The central Zn2+ is tetrahedrally coordinated by two histidines, an aspartate, and a water molecule. The Zn2+ chelating residues occur in the order histidine, aspartate, histidine in all sequences and contact the metal via the N{varepsilon}, the O{delta}, and the N{delta}, respectively. The identity of the other active-site residues varies, but in all enzymes of known structure except for VanX, a conserved histidine is present two residues upstream of the second histidine ligand to the Zn2+. As the same arrangement of active-site residues is also found in the N-terminal, cryptic peptidase domain of sonic hedgehog, we propose that this arrangement of active-site residues be called the "LAS" arrangement, because it is present in lysostaphin-type enzymes, D-Ala-D-Ala metallopeptidases, and in the cryptic peptidase in the N-domain of sonic hedgehog.

Keywords: active site; D-Ala-D-Ala; metallopeptidase; LAS enzymes; lysostaphin; Lyt M

Reprint requests to: Matthias Bochtler, IIMCB, Trojdena 4, 02-109 Warsaw, Poland; e-mail: MBochtler{at}iimcb.gov.pl; fax: 0048-22-6685288.

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.03515704.


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This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Marcyjaniak, S. G. Odintsov, I. Sabala, and M. Bochtler
Peptidoglycan Amidase MepA Is a LAS Metallopeptidase
J. Biol. Chem., October 15, 2004; 279(42): 43982 - 43989.
[Abstract] [Full Text] [PDF]


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