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Protein Science (2004), 13:1331-1339. Published by Cold Spring Harbor Laboratory Press. Copyright © 2004 The Protein Society
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An efficient system for high-level expression and easy purification of authentic recombinant proteins

Ann-Maree Catanzariti1,4, Tatiana A. Soboleva1,5, David A. Jans2,3, Philip G. Board1 and Rohan T. Baker1

1 Molecular Genetics Group and
2 Nuclear Signalling Laboratory, Division of Molecular Medicine, John Curtin School of Medical Research (JCSMR), The Australian National University, Canberra, ACT 0200, Australia
3 Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3168, Australia

(RECEIVED January 8, 2004; FINAL REVISION February 4, 2004; ACCEPTED February 5, 2004)



Abstract

Expression of recombinant proteins as fusions to the eukaryotic protein ubiquitin has been found to significantly increase the yield of unstable or poorly expressed proteins. The benefit of this technique is further enhanced by the availability of naturally occurring deubiquitylating enzymes, which remove ubiquitin from the fusion product. However, the versatility of the system has been constrained due to the lack of a robust, easily purified deubiquitylating enzyme. Here we report the development of an efficient expression system, utilizing the ubiquitin fusion technique, which allows convenient high yield and easy purification of authentic protein. An Escherichia coli vector (pHUE) was constructed for the expression of proteins as histidine-tagged ubiquitin fusions, and a histidine-tagged deubiquitylating enzyme to cleave these fusions was expressed and purified. The expression system was tested using several proteins varying in size and complexity. These results indicate that this procedure will be suitable for the expression and rapid purification of a broad range of proteins and peptides, and should be amenable to high-throughput applications.

Keywords: protein purification; ubiquitin-fusion; deubiquitylating enzyme; affinity purification

Reprint requests to: Rohan T. Baker, Molecular Genetics Group, Division of Molecular Medicine, John Curtin School of Medical Research, The Australian National University, Canberra, ACT 0200, Australia; e-mail: Rohan.Baker{at}anu.edu.au; fax: +61-2-6125-4712.

4 Present addresses: Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra ACT 2601, Australia;

5 Cytokine Molecular Biology and Signalling Group, John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.04618904.


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