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Protein Science (2004), 13:1586-1593. Published by Cold Spring Harbor Laboratory Press. Copyright © 2004 The Protein Society
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Heterodimers of wild-type and subunit interface mutant enzymes of glutathione S-transferase A1–1: Interactive or independent active sites?

Melissa A. Vargo and Roberta F. Colman

Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA

(RECEIVED February 12, 2004; FINAL REVISION March 18, 2004; ACCEPTED March 19, 2004)



Abstract

Heterodimers of rat glutathione S-transferase A1–1 were formed using one wild-type subunit and one subunit with a mutation at the interface to evaluate whether the subunits are interactive or independent. Within the subunit interface, we are considering two regions of interactions: one region consists of a "hydrophobic ball and socket" with Phe 52 from one subunit as the ball and Phe 136 from the second subunit as one of the socket residues. The second region of interaction consists of Arg 69 and Glu 97 from both subunits. The heterodimers were formed after incubation in 1,6-hexanediol. Because one subunit in each pair had a His-tag, the heterodimers were purified using a nickel-nitrilotriacetic acid column. The specific activities of the heterodimer were compared with those of the two homodimers to determine whether the less active, mutant subunit communicates with the other subunit. Two of the heterodimers, wild type/R69E-His and wild type/E97Q-His, displayed specific activities much lower than that expected for independent active sites; in these cases, there are new close repulsive interactions and the low activity of one subunit is communicated to the neighboring subunit. In contrast, the other two heterodimers, wild type/R69Q-His and F136A/wild type-His, exhibited specific activities similar to those expected for independent active sites; in these heterodimers, the closest interaction is not repulsive or occurs over a much longer distance and the subunits act independently. We conclude that whether the subunits interact or are independent depends on the nature of the interactions at the subunit interface.

Keywords: glutathione S-transferase; heterodimers; interface

Abbreviations: GST, glutathione S-transferase • GSH, glutathione • Ni-NTA, nickel-nitrilotriacetic acid • CDNB, 1-chloro-2,4-dinitrobenzene • wild type-His, wild-type enzyme with a six-histidine tag after the initiator methionine. Rat glutathione S-transferase, isozyme 1–1, is designated as the rGSTA1–1 isozyme in the proposed nomenclature by Hayes and Pulford (1995)

Reprint requests to: Roberta F. Colman, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA; e-mail: rfcolman{at}chem.udel.edu; fax: (302) 831-6335.

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.04694004.


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