HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Librizzi, F. Articles by Rischel, C. Search for Related Content PubMed PubMed Citation Articles by Librizzi, F. Articles by Rischel, C. Social Bookmarking                   What's this?

FOR THE RECORD

The kinetic behavior of insulin fibrillation is determined by heterogeneous nucleation pathways

¹ Dipartimento di Scienze Fisiche e Astronomiche dell’Università di Palermo, 90123 Palermo, Italy
² Consiglio Nazionale delle Ricerche—Istituto Nazionale per la Fisica della Materia
³ Niels Bohr Institute, 2100 Copenhagen, Denmark

(RECEIVED July 11, 2005; FINAL REVISION September 21, 2005; ACCEPTED September 27, 2005)

When subjected to acidic conditions and high temperature, insulin is known to produce fibrils that display the common properties of disease amyloids. Thus, clarifying the mechanisms of insulin fibrillation can help the general understanding of amyloidal aggregation. Insulin fibrillation exhibits a very sharp time dependence, with a pronounced lag phase and subsequent explosive growth of amyloidal aggregates. Here we show that the initial stages of this process can be well described by exponential growth of the fibrillated proteins. This indicates that the process is mainly controlled by a secondary nucleation pathway.

Keywords: insulin; protein aggregation; amyloids; nucleation; Thioflavin T; fluorescence

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.051692305.

Reprint requests to: Fabio Librizzi, Dipartimento di Scienze Fisiche e Astronomiche, Via Archirafi 36, 90123 Palermo, Italy; e-mail: librizzi{at}fisica.unipa.it; fax: +39-091-6234281.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Manno, E. F. Craparo, V. Martorana, D. Bulone, and P. L. San Biagio Kinetics of Insulin Aggregation: Disentanglement of Amyloid Fibrillation from Large-Size Cluster Formation Biophys. J., June 15, 2006; 90(12): 4585 - 4591. [Abstract] [Full Text] [PDF]