HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Curtidor, H. Articles by Patarroyo, M. E. Search for Related Content PubMed PubMed Citation Articles by Curtidor, H. Articles by Patarroyo, M. E. Social Bookmarking                   What's this?

Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum erythrocyte binding ligand 1 (EBL-1)-derived peptides

Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Bogotá, Colombia

(RECEIVED August 30, 2004; FINAL REVISION October 4, 2004; ACCEPTED October 9, 2004)

Erythrocyte binding ligand 1 (EBL-1) is a member of the ebl multigene family involved in Plasmodium falciparum invasion of erythrocytes. We found that five EBL-1 high-activity binding peptides (HABPs) bound specifically to erythrocytes: 29895 (⁴¹HKKKSGELNNNKSGILRSTY⁶⁰), 29903 (²⁰¹LYECGK-KIKEMKWICTDNQF²²⁰), 29923 (⁶⁰¹CNAILGSYADIGDIVRGLDV⁶²⁰), 29924(⁶²¹WRDINTNKLSEK-FQKIFMGGY⁶⁴⁰), and 30018 (²⁴⁸¹LEDIINLSKKKKKSINDTSFY²⁵⁰⁰). We also show that binding was saturable, not sialic acid-dependent, and that all peptides specifically bound to a 36-kDa protein on the erythrocyte membrane. The five HABPs inhibited in vitro merozoite invasion depending on the peptide concentration used, suggesting their possible role in the invasion process.

Keywords: malaria protein; erythrocyte binding ligand-1; peptides; Plasmodium falciparum

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.041084305.

Reprint requests to: Hernando Curtidor, Fundación Instituto de Inmunología de Colombia, Carrera 50 No. 26-00, Bogotá, Colombia, 020304 Zona CAN; e-mail: hernando_curtidor{at}fidic.org.co; fax: +57-1-3244672/73 x108.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Forero, A. Puentes, J. Cortes, F. Castillo, R. Vera, L. E. Rodriguez, J. Valbuena, M. Ocampo, H. Curtidor, J. Rosas, et al. Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences that bind specifically to U937 macrophages and A549 epithelial cells Protein Sci., November 1, 2005; 14(11): 2767 - 2780. [Abstract] [Full Text] [PDF]