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1 Department of Chemical Engineering, Princeton University, Princeton, New Jersey 08544, USA
2 Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
(RECEIVED December 18, 2004; FINAL REVISION February 7, 2005; ACCEPTED February 7, 2005)
This work combines two well-established technologies to generate a breakthrough in protein production and purification. The first is the production of polyhydroxybutyrate (PHB) granules in engineered strains of Escherichia coli. The second is a recently developed group of self-cleaving affinity tags based on protein splicing elements known as inteins. By combining these technologies with a PHB-specific binding protein, a self-contained protein expression and purification system has been developed. In this system, the PHB-binding protein effectively acts as an affinity tag for desired product proteins. The tagged product proteins are expressed in E. coli strains that also produce intracellular PHB granules, where they bind to the granules via the PHB-binding tag. The granules and attached proteins can then be easily recovered following cell lysis by simple mechanical means. Once purified, the product protein is self-cleaved from the granules and released into solution in a substantially purified form. This system has been successfully used at laboratory scale to purify several active test proteins at reasonable yield. By allowing the bacterial cells to effectively produce both the affinity resin and tagged target protein, the cost associated with the purification of recombinant proteins could be greatly reduced. It is expected that this combination of improved economics and simplicity will constitute a significant breakthrough in both large-scale production of purified proteins and enzymes and high-throughput proteomics studies of peptide libraries.
Keywords: protein purification; protein expression; polyhydroxybutyrate; intein; self-cleaving affinity tag
Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.041296305.
Reprint requests to: David Wood, Department of Chemical Engineering, Princeton University, A417 Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA; e-mail: dwood{at}princeton.edu; fax: (609) 258-0211.
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