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Protein Science (2005), 14:2236-2245. Published by Cold Spring Harbor Laboratory Press. Copyright © 2005 The Protein Society
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Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines

Javier García, Alvaro Puentes, Luis Rodríguez, Marisol Ocampo, Hernando Curtidor, Ricardo Vera, Ramses Lopez, John Valbuena, Jimena Cortes, Magnolia Vanegas, Carlos Barrero, Manuel A. Patarroyo, Mauricio Urquiza and Manuel E. Patarroyo

Fundación Instituto de Inmunologia de Colombia and the Universidad Nacional de Colombia, Bogotá, Colombia 020304

(RECEIVED April 19, 2005; FINAL REVISION June 1, 2005; ACCEPTED June 13, 2005)

The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.

Keywords: Mycobacterium tuberculosis; high activity binding peptides; receptor–ligand interaction; Mycobacterium tuberculosis complex

Abbreviations: HABP, high activity binding peptide • BS3, bis-sulfosuccinimidyl suberate • HBS, hepes buffer saline • SEC, size exclusion chromatography

Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.051526305.

Reprint requests to: Javier García, Fundación Instituto de Inmunologia de Colombia and the Universidad Nacional de Colombia, Avda. Calle 26 No. 5000, Bogotá, Colombia 020304; e-mail: javgar22{at}hotmail.com; fax: +57-1-4815269.


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J. A. Chapeton-Montes, D. F. Plaza, H. Curtidor, M. Forero, M. Vanegas, M. E. Patarroyo, and M. A. Patarroyo
Characterizing the Mycobacterium tuberculosis Rv2707 protein and determining its sequences which specifically bind to two human cell lines
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[Abstract] [Full Text] [PDF]


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