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Protein Science (2006), 15:2842-2846. Published by Cold Spring Harbor Laboratory Press. Copyright © 2006 The Protein Society
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FOR THE RECORD

Enhanced cell-free protein expression by fusion with immunoglobulin C{kappa} domain

Elizabeth Palmer1, Hong Liu1, Farid Khan1, Michael J. Taussig1, and Mingyue He1

Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom

(RECEIVED July 7, 2006; FINAL REVISION September 8, 2006; ACCEPTED September 25, 2006)

While cell-free systems are increasingly used for protein expression in structural and functional studies, several proteins are difficult to express or expressed only at low levels in cell-free lysates. Here, we report that fusion of the human immunoglobulin {kappa} light chain constant domain (C{kappa}) at the C terminus of four representative proteins dramatically improved their production in the Escherichia coli S30 system, suggesting that enhancement of cell-free protein expression by C{kappa} fusion will be widely applicable.

Keywords: cell-free protein expression; immunoglobulin C{kappa}; domain; fusion protein


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