Protein Science, Vol 3, Issue 10 1697-1705, Copyright © 1994 by Cold Spring Harbor Laboratory Press

INVITED PAPER, SPECIAL SECTION IN HONOR OF MAX PERUTZ

Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors

A. A. KOSSIAKOFF, W. SOMERS, M. ULTSCH, K. ANDOW, Y. A. MULLER and A. M. DE-VOS
Department of Protein Engineering, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080

The crystal structures of complexes of human growth hormone (hGH) with the growth hormone and prolactin receptors (hGHR and hPRLR, respectively), together with the mutational data available for these systems, suggest that an extraordinary combination of conformational adaptability, together with finely tuned specificity, governs the molecular recognition processes operative in these systems. On the one hand, in the active 1:2 ligand-receptor complexes, 2 copies of the same receptor use the identical set of binding determinants to recognize topographically different surfaces on the hormone. On the other hand, comparing the 1:1 hGH-hGHR and hGH-hPRLR complexes, 2 distinct receptors use this same set of binding determinants to interact with the identical binding site on the ligand, even though few residues among the binding determinants are conserved. The structural evidence demonstrates that this versatility is accomplished by local conformational flexibility of the binding loops, allowing adaptation to different binding environments, together with rigid-body movements of the receptor domains, necessary for the creation of specific interactions with the same binding site.
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