Protein Science
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by JAMES, P.
Right arrow Articles by GONNET, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by JAMES, P.
Right arrow Articles by GONNET, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Protein Science, Vol 3, Issue 8 1347-1350, Copyright © 1994 by Cold Spring Harbor Laboratory Press

FOR THE RECORD

Protein identification in DNA databases by peptide mass fingerprinting

P. JAMES, M. QUADRONI, E. CARAFOLI and G. GONNET
Protein Chemistry Laboratory of the Department of Biology, Swiss Federal Institute of Technology (ETH), 8092 Zurich, Switzerland

Proteins can be identified using a set of peptide fragment weights produced by a specific digestion to search a protein database in which sequences have been replaced by fragment weights calculated for various cleavage methods. We present a method using multidimensional searches that greatly increases the confidence level for identification, allowing DNA sequence databases to be examined. This method provides a link between 2-dimensional gel electrophoresis protein databases and genome sequencing projects. Moreover, the increased confidence level allows unknown proteins to be matched to expressed sequence tags, potentially eliminating the need to obtain sequence information for cloning. Database searching from a mass profile is offered as a free service by an automatic server at the ETH, Zurich. For information, send an electronic message to the address cbrg@inf.ethz.ch with the line: help mass search, or help all.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
J.-D. Docquier, T. Lopizzo, S. Liberatori, M. Prenna, M. C. Thaller, J.-M. Frere, and G. M. Rossolini
Biochemical Characterization of the THIN-B Metallo-{beta}-Lactamase of Janthinobacterium lividum
Antimicrob. Agents Chemother., December 1, 2004; 48(12): 4778 - 4783.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Hirsch, K. C. Hansen, A. L. Burlingame, and M. A. Matthay
Proteomics: current techniques and potential applications to lung disease
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L1 - L23.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. H. McDonald, R. Ohi, N. Smelkova, D. Frendewey, and K. L. Gould
Myb-Related Fission Yeast cdc5p Is a Component of a 40S snRNP-Containing Complex and Is Essential for Pre-mRNA Splicing
Mol. Cell. Biol., August 1, 1999; 19(8): 5352 - 5362.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Stenfors, U. Hellman, and J. Silberring
Characterization of Endogenous Neuropeptide Y in Rat Hippocampus and Its Metabolism by Nanospray Mass Spectrometry
J. Biol. Chem., February 28, 1997; 272(9): 5747 - 5751.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by The Protein Society.