Protein Science, Vol 4, Issue 3 361-372, Copyright © 1995 by Cold Spring Harbor Laboratory Press

REVIEWS

Approaches to labeling and identification of active site residues in glycosidases

S. G. WITHERS and R. AEBERSOLD
Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada

Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and biotechnological importance. Knowledge of the identities of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bioengineering of glycosidases with altered characteristics. Here we review and discuss traditional strategies and novel approaches based on tandem mass spectrometry for the identification of the key active site residues in glycosidases.
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