Protein Science
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by RAJAGOPAL, P.
Right arrow Articles by KLEVIT, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by RAJAGOPAL, P.
Right arrow Articles by KLEVIT, R. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Protein Science, Vol 6, Issue 12 2624-2627, Copyright © 1997 by Cold Spring Harbor Laboratory Press

FOR THE RECORD

Demonstration of protein-protein interaction specificity by NMR chemical shift mapping

P. RAJAGOPAL, E. B. WAYGOOD, J. REIZER, M. H. SAIER-JR. and R. E. KLEVIT
University of Washington, Biomolecular Structure Center and Department of Biochemistry, Seattle, Washington 98195-7742

Chemical shift mapping is becoming a popular method for studying protein-protein interactions in solution. The technique is used to identify putative sites of interaction on a protein surface by detecting chemical shift perturbations in simple ((1)H, (15)N)-HSQC NMR spectra of a uniformly labeled protein as a function of added (unlabeled) target protein. The high concentrations required for these experiments raise questions concerning the possibility for non-specific interactions being detected, thereby compromising the information obtained. We demonstrate here that the simple chemical shift mapping approach faithfully reproduces the known functional specificities among pairs of closely related proteins from the phosphoenolpyruvate:sugar phosphotransferase systems of Escherichia coli and Bacillus subtilis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
B. Reichenbach, D. A. Breustedt, J. Stulke, B. Rak, and B. Gorke
Genetic Dissection of Specificity Determinants in the Interaction of HPr with Enzymes II of the Bacterial Phosphoenolpyruvate:Sugar Phosphotransferase System in Escherichia coli
J. Bacteriol., July 1, 2007; 189(13): 4603 - 4613.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. Deutscher, C. Francke, and P. W. Postma
How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria
Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 939 - 1031.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kim, R. Zagozdzon, A. Meisler, J. D. Baleja, Y. Fu, S. Avraham, and H. Avraham
Csk Homologous Kinase (CHK) and ErbB-2 Interactions Are Directly Coupled with CHK Negative Growth Regulatory Function in Breast Cancer
J. Biol. Chem., September 20, 2002; 277(39): 36465 - 36470.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Hashimoto, T. Ikegami, S. Seino, N. Ohuchi, H. Fukada, J. Sugiyama, M. Shirakawa, and T. Watanabe
Expression and Characterization of the Chitin-Binding Domain of Chitinase A1 from Bacillus circulans WL-12
J. Bacteriol., June 1, 2000; 182(11): 3045 - 3054.
[Abstract] [Full Text]

Home page
 Protein Eng Des SelHome page
C. McInnes, S. Grothe, M. O'Connor-McCourt, and B. D. Sykes
NMR study of the differential contributions of residues of transforming growth factor alpha to association with its receptor
Protein Eng. Des. Sel., March 1, 2000; 13(3): 143 - 147.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by The Protein Society.