Protein Science, Vol 7, Issue 11 2472-2475, Copyright © 1998 by Cold Spring Harbor Laboratory Press

FOR THE RECORD

Cation-{pi} (Na(+)-Trp) interactions in the crystal structure of tetragonal lysozyme

J. WOUTERS
Facultes Universitaires N.-D. de la Paix, 61 Rue de Bruxelles, B-5000 Namur, Belgium

Experimental evidence of a cation-{pi} interaction between a sodium cation (Na(+)) and the indole ring of residue Trp123 in a structure (2.0 A) of hen egg-white lysozyme is presented. The geometry of the metal ion-{pi} interaction observed in the protein structure (distance between the aromatic plane and the cation ~4 A) is consistent with geometries observed among small molecules crystal structures and quantum chemistry ab initio calculations. The present crystal structure of lysozyme provides unique structural information about the geometry of binding of cations to {pi} systems in proteins. It shows that the metal ion-{pi} interaction within proteins is not significantly different from similar bindings found in small molecules and that it can be modeled by theoretical methods.
CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Wouters, Y. Oudjama, S. J. Barkley, C. Tricot, V. Stalon, L. Droogmans, and C. D. Poulter Catalytic Mechanism of Escherichia coli Isopentenyl Diphosphate Isomerase Involves Cys-67, Glu-116, and Tyr-104 as Suggested by Crystal Structures of Complexes with Transition State Analogues and Irreversible Inhibitors J. Biol. Chem., March 28, 2003; 278(14): 11903 - 11908. [Abstract] [Full Text] [PDF]

				D. R. Hall, C. S. Bond, G. A. Leonard, C. I. Watt, A. Berry, and W. N. Hunter Structure of Tagatose-1,6-bisphosphate Aldolase. INSIGHT INTO CHIRAL DISCRIMINATION, MECHANISM, AND SPECIFICITY OF CLASS II ALDOLASES J. Biol. Chem., June 7, 2002; 277(24): 22018 - 22024. [Abstract] [Full Text] [PDF]

				J. Hu, L. J. Barbour, and G. W. Gokel Supramolecular Chemistry And Self-assembly Special Feature: Probing alkali metal-pi interactions with the side chain residue of tryptophan PNAS, April 16, 2002; 99(8): 5121 - 5126. [Abstract] [Full Text] [PDF]

				S. L. De Wall, E. S. Meadows, L. J. Barbour, and G. W. Gokel Synthetic receptors as models for alkali metal cation-pi binding sites in proteins PNAS, June 6, 2000; 97(12): 6271 - 6276. [Abstract] [Full Text] [PDF]

				J. P. Gallivan and D. A. Dougherty Cation-pi interactions in structural biology PNAS, August 17, 1999; 96(17): 9459 - 9464. [Abstract] [Full Text] [PDF]

				M. Alvarez, J. Wouters, D. Maes, V. Mainfroid, F. Rentier-Delrue, L. Wyns, E. Depiereux, and J. A. Martial Lys13 Plays a Crucial Role in the Functional Adaptation of the Thermophilic Triose-phosphate Isomerase from Bacillus stearothermophilus to High Temperatures J. Biol. Chem., July 2, 1999; 274(27): 19181 - 19187. [Abstract] [Full Text] [PDF]

				J. Hu, L. J. Barbour, and G. W. Gokel Supramolecular Chemistry And Self-assembly Special Feature: Probing alkali metal-pi interactions with the side chain residue of tryptophan PNAS, April 16, 2002; 99(8): 5121 - 5126. [Abstract] [Full Text] [PDF]