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Protein Science, Vol 7, Issue 2 457-469, Copyright © 1998 by Cold Spring Harbor Laboratory Press

ARTICLE

A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry

P. T. JEDRZEJEWSKI, A. GIROD, A. THOLEY, N. KONIG, S. THULLNER, V. KINZEL and D. BOSSEMEYER
Present address: The Barnett Institute, Northeastern University, 341 Mugar Building, 360 Huntington Avenue, Boston, Massachusetts 02115.

The N-terminal sequence myr-Gly-Asn is conserved among the myristoylated cAPK (protein kinase A) catalytic subunit isozymes C{alpha}, C{beta}, and C{gamma}. By capillary LC-MS and tandem MS, we show that, in approximately one third of the C{alpha} and C{beta} enzyme populations from cattle, pig, rabbit, and rat striated muscle, Asn 2 is deamidated to Asp 2. This deamidation accounts for the major isoelectric variants of the cAPK C-subunits formerly called C(A) and C(B). Deamidation also includes characteristic isoaspartate isomeric peptides from C{alpha} and C{beta}. Asn 2 deamidation does not occur during C-subunit preparation and is absent in recombinant myristoylated C{alpha} (rC{alpha}) from Escherichia coli. Deamidation appears to be the exclusive pathway for introduction of an acidic residue adjacent to the myristoylated N-terminal glycine, verified by the myristoylation negative phenotype of an rC{alpha}(Asn 2 Asp) mutant. This is the first report thus far of a naturally occurring myr-Gly-Asp sequence. Asp 2 seems to be required for the well-characterized (auto)phosphorylation of the native enzyme at Ser 10. Our results suggest that the myristoylated N terminus of cAPK is a conserved site for deamidation in vivo. Comparable myr-Gly-Asn sequences are found in several signaling proteins. This may be especially significant in view of the recent knowledge that negative charges close to myristic acid in some proteins contribute to regulating their cellular localization.
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R. Pepperkok, A. Hotz-Wagenblatt, N. Konig, A. Girod, D. Bossemeyer, and V. Kinzel
Intracellular Distribution of Mammalian Protein Kinase A Catalytic Subunit Altered by Conserved Asn2 Deamidation
J. Cell Biol., February 21, 2000; 148(4): 715 - 726.
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