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Protein Science, Vol 7, Issue 4 1039-1045, Copyright © 1998 by Cold Spring Harbor Laboratory Press

ARTICLE

Complete localization of disulfide bonds in GM2 activator protein

C. G. SCHUTTE, T. LEMM, G. J. GLOMBITZA and K. SANDHOFF
Kekule-Institut fur Organische Chemie und Biochemie der Universitat Bonn, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany Both authors contributed equally to this work.

Lysosomal degradation of ganglioside GM2 by hexosaminidase A requires the presence of a small, non-enzymatic cofactor, the GM2-activator protein (GM2AP). Lack of functional protein leads to the AB variant of GM2-gangliosidosis, a fatal lysosomal storage disease. Although its possible mode of action and functional domains have been discussed frequently in the past, no structural information about GM2AP is available so far. Here, we determine the complete disulfide bond pattern of the protein. Two of the four disulfide bonds present in the protein were open to classical determination by enzymatic cleavage and mass spectrometry. The direct localization of the remaining two bonds was impeded by the close vicinity of cysteines 136 and 138. We determined the arrangement of these disulfide bonds by MALDI-PSD analysis of disulfide linked peptides and by partial reduction, cyanylation and fragmentation in basic solution, as described recently (Wu F, Watson JT, 1997, Protein Sci 6:391-398).
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