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Protein Science, Vol 7, Issue 8 1811-1820, Copyright © 1998 by Cold Spring Harbor Laboratory Press

ARTICLE

Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents

F. DONATE, A. ARTIGUES, A. IRIARTE and M. MARTINEZ-CARRION
Present address: Immunology Department, The Scripps Research Institute, La Jolla, California 92037.

GroEL has a greater affinity for the mitochondrial isozyme (mAAT) of aspartate aminotransferase than for its cytosolic counterpart (cAAT) (Mattingly JR Jr, Iriarte A, Martinez-Carrion M, 1995, J Biol Chem 270:1138-1148), two proteins that share a high degree of sequence similarity and an almost identical spatial structure. The effect of detergents on the refolding of these large, dimeric isozymes parallels this difference in behavior. The presence of non-ionic detergents such as Triton X-100 or lubrol at concentrations above their critical micelle concentration (CMC) interferes with reactivation of mAAT unfolded in guanidinium chloride but increases the yield of cAAT refolding at low temperatures. The inhibitory effect of detergents on the reactivation of mAAT decreases progressively as the addition of detergents is delayed after starting the refolding reaction. The rate of disappearance of the species with affinity for binding detergents coincides with the slowest of the two rate-limiting steps detected in the refolding pathway of mAAT. Limited proteolysis studies indicate that the overall structure of the detergent-bound mAAT resembles that of the protein in a complex with GroEL. The mAAT folding intermediates trapped in the presence of detergents can resume reactivation either upon dilution of the detergent below its CMC or by adding {beta}-cyclodextrin. Thus, isolation of otherwise transient productive folding intermediates for further characterization is possible through the use of detergents.
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F. Donate, A. J. Yanez, A. Iriarte, and M. Martinez-Carrion
Interaction of the Precursor to Mitochondrial Aspartate Aminotransferase and Its Presequence Peptide with Model Membranes
J. Biol. Chem., October 27, 2000; 275(44): 34147 - 34156.
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