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Protein Science, Vol 9, Issue 1 194-196, Copyright © 2000 by The Protein Society

JOURNAL ARTICLE

Kinetic evidence of an on-pathway intermediate in the folding of lysozyme

Y Bai
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20850, USA. yawen@helix.nih.gov

By means of a kinetic test, it was demonstrated that one of the folding intermediates (Ialpha) of hen lysozyme with alpha-domain folded and beta-domain unfolded is on the folding pathway under the classical definition. Ialpha folds to the native (N) state directly (unfolded (U) <==> Ialpha <==> N) without having to unfold to U and then refold to N through alternative folding pathways as in Ialpha <==> U <==> N.
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