Protein Science
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luck, L. A.
Right arrow Articles by Johnson, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luck, L. A.
Right arrow Articles by Johnson, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Protein Science, Vol 9, Issue 12 2573-2576, Copyright © 2000 by Cold Spring Harbor Laboratory Press

JOURNAL ARTICLE

Fluorescence and 19F NMR evidence that phenylalanine, 3-L-fluorophenylalanine and 4-L-fluorophenylalanine bind to the L-leucine specific receptor of Escherichia coli

LA Luck and C Johnson
Department of Chemistry, Clarkson University, Potsdam, New York 13699, USA. luckla@clarkson.edu

The binding capacity of the L-leucine receptor from Escherichia coli was measured with L-phenylalanine and 4-fluoro-L-phenylalanine as substrates by fluorescence. The apparent dissociation constants (KD) for L-leucine, L-phenylalanine, and 4-fluoro-L-phenylalanine are 0.40, 0.18, and 0.26 respectively. 19F NMR data show protein-induced shifts for the 4-fluoro-L-phenylalanine peak and 3-fluoro-L-phenylalanine when receptor is present. Evidence points to the binding of only the L-isomers of these fluorine analogs.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
U. Magnusson, B. Salopek-Sondi, L. A. Luck, and S. L. Mowbray
X-ray Structures of the Leucine-binding Protein Illustrate Conformational Changes and the Basis of Ligand Specificity
J. Biol. Chem., March 5, 2004; 279(10): 8747 - 8752.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. L. Grage, J. Wang, T. A. Cross, and A. S. Ulrich
Solid-State 19F-NMR Analysis of 19F-Labeled Tryptophan in Gramicidin A in Oriented Membranes
Biophys. J., December 1, 2002; 83(6): 3336 - 3350.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
B. Salopek-Sondi and L. A. Luck
19F NMR study of the leucine-specific binding protein of Escherichia coli: mutagenesis and assignment of the 5-fluorotryptophan-labeled residues
Protein Eng. Des. Sel., November 1, 2002; 15(11): 855 - 859.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by The Protein Society.