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Protein Science, Vol 9, Issue 3 435-439, Copyright © 2000 by The Protein Society
JOURNAL ARTICLE |
WJ van Berkel, RH van den Heuvel, C Versluis and AJ Heck
Department of Biomolecular Sciences, Wageningen University, The Netherlands.
Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%. The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.
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