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Protein Science, Vol 9, Issue 4 755-757, Copyright © 2000 by The Protein Society

JOURNAL ARTICLE

Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion

E Saridakis and NE Chayen
Division of Biomedical Sciences, Imperial College of Science, Technology and Medicine, London, United Kingdom.

A simple method for growing protein crystals in the metastable zone using the vapor diffusion technique is described. The coverslips holding the hanging drops are transferred, after being incubated for some time at conditions normally giving many small crystals, over reservoirs at concentrations that normally yield clear drops. Fewer, much larger and better diffracting crystals are obtained, compared with conventional crystallization at similar conditions. To our knowledge, this is the first report of a significant crystal improvement due to "backing off" from nucleation conditions, using the hanging drop method. A correlation of the transfer time with published results for vapor diffusion equilibration of poly(ethylene glycol) solutions is also presented.
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 Biophys. JHome page
E. Saridakis and N. E. Chayen
Systematic Improvement of Protein Crystals by Determining the Supersolubility Curves of Phase Diagrams
Biophys. J., February 1, 2003; 84(2): 1218 - 1222.
[Abstract] [Full Text] [PDF]


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