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Fig. 4. Temperature dependence of the CD intensity for CaVP, N-CaVP, and C-CaVP at pH 7.5. (A) CaVP in the presence of 1 mM EGTA at 291 (thin dotted line) and 222 nm (thick dotted line) and in the presence of 2 mM CaCl2 at 291 (thin solid line) and 222 nm (thick solid line). (B) N-CaVP at 222 nm (thick line) and 291 nm (thin line). (C) C-CaVP at 222 nm in the presence of 1 mM EGTA. The inset shows the first derivative of the CD melting curve.
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