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1 Department of Biochemistry, Lund University, S-221 00 Lund, Sweden
2 Department of Biochemistry, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
Reprint requests to: Dr. Cecilia Sundby Emanuelsson, Department of Biochemistry, Lund University, P.O. Box 124, S-221 00 Lund, Sweden; e-mail: Cecilia{at}biochem.lu.se; fax: 46 46 222 4534.
(RECEIVED March 23, 2001; FINAL REVISION May 30, 2001; ACCEPTED June 4, 2001)
Article and publication are at http://www.proteinscience.org/cgi/doi/10.1101/ps.11301.
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Abstract |
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-helix with the methionines situated along one side. The functional role of these conserved methionines is not understood. We have found previously that treatment, which causes methionine sulfoxidation in Hsp21, also leads to structural changes and loss of chaperone-like activity. Here, mutants of Arabidopsis thaliana Hsp21 protein were created by site-directed mutagenesis, whereby conserved methionines were substituted by oxidation-resistant leucines. Mutants lacking the only cysteine in Hsp21 were also created. Protein analyses by nondenaturing electrophoresis, size exclusion chromatography, and circular dichroism proved that sulfoxidation of the four highly conserved methionines (M49, M52, M55, and M59) is responsible for the oxidation-induced conformational changes in the Hsp21 oligomer. In contrast, the chaperone-like activity was not ultimately dependent on the methionines, because it was retained after methionine-to-leucine substitution. The functional role of the conserved methionines in Hsp21 may be to offer a possibility for redox control of chaperone-like activity and oligomeric structure dynamics. Keywords: Chaperone-like activity; methionine sulfoxidation; redox-response; small heat shock protein
Abbreviations: CD, circular dichroism • DTT, dithiothreitol • H2O2, hydrogen peroxide • MALDI/TOF, matrix assisted laser desorption ionization/time of flight • PAGE, polyacrylamide gel electrophoresis • SDS, sodium lauryl sulfate • sHsp, small heat shock protein
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Introduction |
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100 amino acids and a variable N-terminal domain (Caspers et al. 1995; de Jong et al. 1998). Different roles have been suggested for sHsps, such as regulation of cytoskeleton protein dynamics (Lavoie et al. 1993; Wang and Spector 1996) and regulation of apoptosis (Mehlen et al. 1997). All sHsps are known to show a non-ATP-dependent, chaperone-like activity in vitro, which was first shown for B-crystallin (Horwitz 1992). The sHsps protect other proteins from aggregation at elevated temperatures, by binding their molten globule forms to their outer surface (Lindner et al. 1997, 1998). When the temperature decreases, the protected proteins are released, probably assisted by ATP-dependent Hsp70 chaperones (Lee and Vierling 2000).
Interestingly, the variable N-terminal domain of the chloroplast-localized sHsp, Hsp21, contains a highly conserved methionine-rich sequence (Waters et al. 1996). Secondary structure predictions indicate that this methionine-rich sequence can form an amphipathic -helix with all the methionines exposed on one side (Chen and Vierling 1991). The conservation of the methionines in this domain among divergent plant species indicates that it is of functional importance, but the actual function of Hsp21 in the chloroplast, as well as the role of the highly conserved methionines, is not understood.
Like other sHsps, Hsp21 is an oligomeric protein, but the actual number of subunits per oligomer is not known. The apparent molecular mass can be estimated by gel filtration or native PAGE to be approximately 400 kD. We have found previously that the Arabidopsis thaliana Hsp21 oligomer undergoes conformational changes in response to oxidation, coinciding with and probably being caused by sulfoxidation of the methionine residues in the conserved amphipathic helix (Gustavsson et al. 1999). This oxidation-induced conformational change of Hsp21 occurs concomitantly with a loss of the chaperone-like activity and a decrease in -helical secondary structure (Härndahl et al. 2001).
All amino acids that occur naturally in proteins can be modified oxidatively, but the two most readily oxidized are the sulfur-containing cysteine and methionine (Berlett and Stadtman 1997). Cysteine oxidation, which can lead to a variety of products such as the cysteic acid or formation of disulfide bridges with another cysteine residue, is the best characterized of the two. Because of the normally reducing intracellular environment, disulfide bridges are not as common, and their formation in response to, for example, oxidative stress often leads to loss of protein function, which can be regained by reduction of the disulfide bridge. Oxidation of methionine to the methionine sulfoxide leads to a drastic decrease in hydrophobicity and to a more rigid structure of the side chain. There are numerous reports about how methionine sulfoxidation, like disulfide bridge formation between cysteines, leads to loss of protein function (Gao et al. 1998; Johnson and Travis 1979; Vogt 1995). Similar to the example of disulfide bridge formation, methionine sulfoxidation is a reversible reaction. The enzyme responsible for reduction of methionine sulfoxides, the peptide methionine sulfoxide reductase (MsrA), is found in various organisms ranging from bacteria to plants and mammals (Moskovitz et al. 1995, 1996; Sadanandom et al. 2000). In this paper we have applied a site-directed mutagenesis approach to assess the importance of the conserved methionine residues in Hsp21 and the involvement of methionine sulfoxidation in the oxidation-induced structural changes and chaperone-like activity of the Arabidopsis thaliana Hsp21 oligomer.
Mutant Hsp21 protein with methionines substituted by leucines was therefore prepared. Methionine and leucine are hydrophobic and resemble each other structurally. Also, in substitution mutations during evolution, methionine is most frequently replaced by leucine. However, the leucine side chain contains no sulphur atom and is not readily oxidized. To investigate the possible contribution of cysteine oxidation in oxidation-induced structural changes, the single cysteine residue (C151) in Arabidopsis thaliana was replaced by alanine. The resulting set of mutant proteins with different substitutions was evaluated and showed that methionines substitution clearly abolished the oxidation-induced conformational changes of the Hsp21 oligomer, whereas cysteine substitution gave no effect in this respect. The chaperone-like activity of Hsp21 was largely retained in leucine-containing Hsp21 mutants. The data obtained in this study indicate that the methionine-rich Hsp21, which evolved during land-plant evolution (Waters and Vierling 1999), presumably coevolved with a highly reducing environment in the chloroplast.
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-helix contains four methionines (M49, M52, M55, and M59) that are highly conserved in most land plants (M49, M52, and M59: conserved in 10 out of 10 species examined; M55: nine out of 10 [data not shown]) and two methionines (M62 and M67) that are less well conserved (M62: two out of 10; M67: five out of 10). Another methionine residue is found at position 35 in the N-terminal part of the Arabidopsis thaliana Hsp21 sequence and is conserved in only two of the 10 species examined. There are also two additional highly conserved methionines, M97 and M101 (both 10 out of 10), which are not located in the N-terminal domain but in the structurally ordered C-terminal domain. The two conserved methionines in this C-terminal domain are less prone to methionine sulfoxidation, although all methionines in Hsp21 can be oxidized into methionine sulfoxides upon treatment with hydrogen peroxide as detected by mass spectrometry (Gustavsson et al. 1999). In the structure that was resolved for Methanococcus jannaschii Hsp16 (Kim et al. 1998), another sHsp-like chaperone, the N-terminal region was disordered and therefore not seen in the structure. This indicates that the N-terminal region is flexible. Thus in the case of Hsp21, the amphipathic -helix in the N-terminal region may act as a flexible arm and respond to redox changes conducted via methionine sulfoxidation of the six methionines, thereby affecting the conformation of the Hsp21 oligomer.
To evaluate how the methionines in this flexible part of Hsp21 are involved in the conformational changes in Hsp21 observed in response to oxidation and how the methionines affect the chaperone-like activity, mutants with either the four most highly conserved or all six methionines in the amphipathic -helix were substituted for leucines by site-directed mutagenesis. These mutants, Hsp21-M(49,52,55, 59)L and Hsp21-M(49,52,55,59,62,67)L, are referred to as -4M, and -6M. Two other mutants were made with the only cysteine in Hsp21 replaced by alanine: Hsp21-C151A and Hsp21-M(49,52,55,59,62,67)L,C151A, referred to as -C and -6M-C. The mutations were verified at the level of the expressed proteins using MALDI/TOF mass spectrometry on peptides obtained by proteolysis and at the DNA level by sequencing of the coding regions of the plasmids (data not shown). The DNA sequencing verified that no unwanted mutations had occurred during the PCR reactions.
Methionines required for conformational change in response to oxidation
To evaluate if the oxidation-induced conformational change reported previously for Hsp21 (Gustavsson et al. 1999) can occur in the mutants, samples of purified wild-type and mutant Hsp21 protein were oxidized with 5 or 10 mM hydrogen peroxide and subjected to nondenaturing PAGE as shown in Figure 1
. For wild-type Hsp21, the conformational change is seen as a shift of the 400 kD band into an upper band at 450 kD in response to oxidation with either 5 or 10 mM hydrogen peroxide. For the mutants in which four or six methionines are replaced by leucines (-4M, -6M), there is no shift into an upper band. The Hsp21 oligomer forms a major band at 400 kD. Additional weak extra bands can also be seen, which could be misfolded oligomers or degraded oligomers, but the main band still accounts for some 95% of the protein for the -6M mutant. The mutant with the one cysteine removed (-C) behaves like the wild type, showing a shift of the 400 kD band into an upper band at 450 kD in response to oxidation with either 5 or 10 mM hydrogen peroxide. Thus, the shift into the upper band indicating a change in oligomeric conformation of Hsp21 is because of sulfoxidation of the conserved methionines, not cysteine oxidation. The mutant in which the six methionines and the cysteine were replaced by leucines and alanine, respectively, (-6M-C) behaved like the -4M and -6M mutants.
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shows size exclusion chromatograms for (Fig. 2A) nonoxidized and (Fig. 2B) oxidized samples of wild-type and the mutant forms of Hsp21. As seen for the wild-type Hsp21 oligomer, a change in apparent molecular weight occurred upon oxidation from 400 kD to smaller forms, predominantly one of 100 kD (indicated with asterisk), with part of the 400 kD peak still remaining. However, the mutants in which methionines were replaced by leucines (-4M, -6M, and -6M-C) all appeared unaffected by the oxidation. In case of the -C mutant, the 400 kD peak was broadened after oxidation but without the appearance of the 100 kD form. Thus, it appears that it is cysteine oxidation in the wild-type Hsp21 that makes the oligomer fall apart, but methionine sulfoxidation in the amphipathic helix is the prerequisite for any conformational change.
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). The 70°C sample of wild-type Hsp21 oligomer resembled the control, whereas in the 90°C sample, the band appeared weaker because of formation of aggregates that do not enter the gel. A similar pattern was observed for the -4M and -6M mutants with somewhat less aggregation. However, both mutants in which the cysteine had been replaced (-C, and -6M-C) appeared unaffected by heat treatment at 90°C. These data show that cysteine-151, one in each monomer, is crucial for the aggregation of the Hsp21 oligomer that occurs at very high temperatures.
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-helical CD signal-helical secondary structure, which did not occur in the Hsp21 -6M mutant (Härndahl et al. 2001). Hence, the Hsp21 -4M and -C mutants were also screened by CD to record their response to oxidation in terms of the 222 nm signal (indicated by arrows in Fig. 4) from the methionine-rich amphipathic -helix. An oxidation-induced decrease in 222 nm signal was seen for wild-type Hsp21 (Fig. 4A) and in the Hsp21 mutant in which the cysteine was replaced (Fig. 4C). However, such a decrease in the 222 nm CD signal was totally lacking in the Hsp21 mutant without the four most conserved (-4M) methionines in the amphipathic -helix (Fig. 4B). Thus, methionine sulfoxidation of the four most conserved methionine residues (M49, M52, M55, and M59) is responsible for the conformational change which causes loss of -helical secondary structure with no contribution of cysteine oxidation. Oxidation of only the two semiconserved methionines, M62 and M67, in the -4M mutant is obviously not sufficient to cause loss in the -helical secondary structure.
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, the -6M mutant showed even better chaperone-like activity than wild-type Hsp21. Furthermore, as shown in the insert, the chaperone-like activity of -6M was unaffected by oxidation. In the insulin assay (Fig. 5B), the -6M mutant has less chaperone-like activity than wild-type Hsp21, although it still shows good chaperone-like activity. In both assays, the mutant with four methionines replaced by leucine (-4M) behaved in a way similar to -6M (data not shown). The mutant from which the six methionines and the cysteine had been removed (-6M-C) showed very little chaperone-like activity (data not shown) for reasons we cannot explain at this point. It is possible that this mutant has a less stable tertiary structure (see below).
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) with the total tryptophan fluorescence (calculated by integration of fluorescence between 315 and 420 nm) at increasing urea concentrations (Fig. 6B), we could analyze the conformational stability of the Hsp21 oligomer. In a first phase at very low urea concentrations (Fig. 6A [I]), Hsp21 is still intact as an oligomer. When it reaches a certain urea concentration, the oligomer starts to disassemble, which is accompanied by a rapid decrease in fluorescence intensity (Fig. 6B). Early during this second phase (II) at intermediate urea concentrations, fluorescence becomes red shifted (peak maximum shifted from 340 to 358 nm) as unfolding of the Hsp21 monomer starts (data not shown). At higher urea concentrations (III), the red-shifted fluorescence increases continuously during the gradual unfolding.
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, total fluorescence is presented, showing that conformational stability is higher in the Hsp21 mutants with four or all six methionines replaced by leucine (squares and triangles, respectively) compared to wild-type Hsp21 (circles). In the mutant Hsp21 proteins, the initial fluorescence level characteristic of oligomeric Hsp21 was completely retained in 0.5 M urea, which was the approximate midpoint of the fluorescence decrease for wild-type Hsp21 protein. This indicates a more stable oligomeric conformation for the methionine-less mutant forms compared to wild-type Hsp21. Furthermore, in both the mutants, the red shift was delayed until higher urea concentrations: The red shift of the fluorescence peak maximum from 340 to 358 nm did not occur until 2.5 M urea, whereas in wild-type Hsp21, the red shift started at 1 M urea (data not shown). Fluorescence from the mutant from which cystein had been removed (-C) was similar to wild-type Hsp21, whereas the -6M-C mutant deviated from all the other Hsp21 proteins, showing a higher fluorescence intensity in the absence of urea, which indicated an altered tryptophan environment, and a fluorescence increase in 0.5 M urea (data not shown). The additive effect of methionine and cystein replacement thus results in an altered tertiary structure of the -6M-C mutant protein, which could also explain its poor chaperone-like activity, which was discussed above.
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Discussion |
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-helix replaced by leucines lacked response to oxidation in terms of conformational change in the Hsp21 oligomer as judged by nondenaturing PAGE and size exclusion chromatography (Figs. 1, 2
). These results are consistent with the finding that loss of -helical secondary structure upon oxidation (Fig. 4), did not occur unless the four most conserved methionine residues (M49, M52, M55, and M59) were present. Thus, the redox-response of the Hsp21 oligomer, reported here as a conformational change in the Hsp21 oligomer and loss of -helical secondary structure, which previously was shown to correlate well with methionine sulfoxidation (Gustavsson et al. 1999), indeed depend on sulfoxidation of the four most conserved methionine residues with no involvement of cysteine oxidation.
Chaperone-like activity was unaffected by oxidation when the six methionines were replaced by leucines in the -6M Hsp21 mutant (Fig. 5A, insert). Remarkably, in the citrate synthase assay, the -6M Hsp21 mutant also showed even better chaperone-like activity than wild-type Hsp21 (Fig. 5A). In the insulin assay, however, its relative activity compared to the wild-type Hsp21 was not as good (Fig. 5B). In both assays the chaperone-like activity of the -6M Hsp21 mutant was of the same order of magnitude as the well-established chaperone-like activity of B-crystallin (Horwitz 1992; Lindner et al. 1997, 1998; van Boekel et al. 1999), but in the insulin assay, the chaperone-like activity of wild-type Hsp21 was even better than that of B-crystallin. The presence of DTT in the insulin assay may resemble the reducing conditions which prevail in the chloroplast, to which Hsp21 probably has adapted to function optimally. In several reports on proteins, which contain functionally important methionines, substitution leads to loss in efficiency (Yuan and Vogel 1999). Here, an apparent improvement of the chaperone-like activity was obtained for the -6M Hsp21 mutant in the DTT-less CS assay in which there is probably a "background" methionine sulfoxidation in the wild-type Hsp21. In none of the assays did replacement of methionines by leucines in Hsp21 cause a major decrease in chaperone-like activity.
The mutant-lacking C151 behaved similarly to wild-type Hsp21 except at unphysiological denaturating temperatures (70°C and 90°C) in which it was less aggregation-prone, indicating that such temperature-induced aggregation involves cysteine oxidation. The C151 may reside in the interphase between subunits in the Hsp21 oligomer as judged by sequence alignment with the Methanococcus jannaschii Hsp16 structure (Kim et al. 1998). This could explain why wild-type Hsp21 but not C151A yielded 100 kD tetramers after methionine sulfoxidation (Fig. 2). But under physiological conditions, oxidation of cystein 151 is probably less important. The cysteine residue C151 in Arabidopsis thaliana Hsp21 is nonconserved, and other plant species contain either several cystein residues (e.g., pea [Pisum sativum]) or no cysteines at all. In Hsp25 disulfide bond formation did not affect secondary structure, degree of oligomerization, or chaperone activity (Zavialov et al. 1998).
Even if the leucine-substituted form of Hsp21 shows functional chaperone-activity in vitro, the methionines may be important for other in vivo functions of Hsp21. When the methionine side chain undergoes oxidation, which the leucine side chain is unable to, its hydrophobicity is markedly decreased, together with a decrease in structural flexibility. If the methionine-rich region of Hsp21 is involved in the chaperone-like activity, oxidation of the methionines would most likely affect the binding of the substrate proteins. The methionine sulfoxide-containing conformationally changed form of Hsp21, which has lost chaperone-like activity (Härndahl et al. 2001), may fulfill another, yet unknown role in the cell. It may, for example, bind to components other than the reduced form of Hsp21, for which chaperone-like activity might be the main functional role. Such redox-dependent regulation of Hsp21 would be an alternative pathway to regulate its function, parallel to, for example, phosphorylation of other sHsps. Phosphorylated forms of the mammalian Hsp27 show no chaperone-like activity (Rogalla et al. 1999), but bind cytoskeletal elements (Zhu et al. 1994).
Replacement of methionines by leucines did not decrease, but rather increased the conformational stability of Hsp21 oligomer stability (Fig. 7). The midpoint of the urea-induced oligomer disassembly (fluorescence decrease) was clearly affected in the mutants: 0.5 M urea for wild-type Hsp21, but approximately 1.5 M urea for both Hsp21 mutants (-4M and -6M). Also, the fluorescence red shift typical for unfolding occurred at much lower urea concentration in the wild-type Hsp21 (1 M urea) compared to the mutants (2.5 M urea). These results indicate that substitution of leucines by methionines during evolution decreased conformational stability. The oxidizable methionines in Hsp21 were maybe allowed during land plant evolution (Waters and Vierling 1999) only since the chloroplast represented a safe reducing environment in which methionines in Hsp21 can be kept in a reduced state. Therefore, one should expect land plant evolution to coincide with the evolution of factors that contribute to the reduced environment: NADPH-producing Photosystem II, and redox-controlling systems like thioredoxin, glutaredoxin, and ascorbate peroxidase.
Thus, methionines may also have evolved in Hsp21 to allow regulation of different functions of Hsp21. In a reducing environment, the chaperone function might be the main task for the large oligomeric form of Hsp21, whereas during oxidative stress, the smaller 100 kD forms caused by oxidation-induced disassembly of Hsp21 may have another function. Of the four methionines conserved among angiosperms (M49, M52, M55, and M59), only one is present in the moss Funario hygrometrica (Waters and Vierling 1999), although secondary predictions indicate an -helical secondary structure. Thus, the possibility for redox regulation of chaperone-like activity as in Hsp21 may be missing in evolutionary "early" organisms. In this context it should be mentioned that another type of regulation, which is fairly well characterized — phosphorylation of the D1-protein in Photosystem II — is a late evolutionary event that occurs in seed plants but not in mosses, liverworts, and ferns (Pursiheimo et al. 1998). Transgenic Arabidopsis plants expressing the leucine-substituted form of Hsp21 could shed further light on whether the leucine-substituted form of Hsp21 is fully functional or if regulation of Hsp21 by methionine sulfoxidation is needed and is of physiological importance.
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Materials and methods |
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Site-directed mutagenesis
Mutagenesis of the expression vector pAZ376, encoding the mature form of Arabidopsis thaliana Hsp21, was performed using the QuickChange Site-Directed Mutagenesis Kit (Stratagene). For the mutagenesis of methionines 49, 52, and 55 to leucines, the primer 5'-GGAAACAGTGGTGACTCCTGCGACGCTGTTGACAATC TATGATACC-3' and its corresponding antisense primer were used, leading to plasmid pAZ376-M(49,52,55)L. Annealing temperature was 50°C. Using pAZ376-M(49,52,55)L as template and primer 5'-CGCTGTTGACAATCTATGGGACCTGTCCTACAA GC-3' and its corresponding antisense primer, a mutant with methionines 49, 52, 55, and 59 mutated to leucines was made, leading to plasmid pAZ376-M(49,52,55,59)L. Annealing temperature was 55°C. Using pAZ376-M(49,52,55,59)L as template and primer 5'-GGGACCTGTCCGACAAGCTCCTGTGAGACGGA CAGAGTCC-3' and its corresponding antisense primer, a mutant with methionines 49, 52, 55, 59, 62, and 67 mutated to leucines was made, leading to plasmid pAZ376-M(49,52,55,59,62,67)L. Annealing temperature was 50°C. For the mutagenesis of cysteine 151 to alanine in pAZ376 and pAZ376-M(49,52,55,59,62,67)L, primer 5'-CGAGGGTCTGTTGCGACTCTTTCTGTTCTAG-3' and its corresponding antisense primer were used, leading to plasmids pAZ376-C151A and pAZ376-M(49,52,55,59,62,67)L, C151A. Annealing temperature was 52°C. Underlined nucleotides show the mutated codons.
Expression and purification of mutants
The plasmids pAZ376-M(49,52,55,59)L, pAZ376-M(49,52,55,59, 62,67)L, pAZ376-M(49,52,55,59,62,67)L,C151A, and pAZ376-C151A were transformed into competent Escherichia coli BL21 (DE3) cells and grown at 37°C until midlog phase in Luria-Bertani (LB) broth containing 0.2 mg/mL of ampicillin. Isopropyl ß-D-thiogalactopyranoside (IPTG) was then added to a final concentration of 0.5 mM, and incubation was continued for 4 h. Pure mutant Hsp21 was prepared essentially as described earlier (Härndahl et al. 1998) using only size exclusion chromatography in the final purification step.
Size exclusion chromatography
Size exclusion chromatography was performed using a Pharmacia HiLoad/Superdex 200 HR 16/60 column at 4°C. Equilibration and elution was performed with 0.1 M ammonium bicarbonate at pH 7.8 or 25 mM Tris-HCl at pH 7.0 at a flow rate of 0.75 mL/min.
Oxidation or heat treatment of purified Hsp21
Purified recombinant wild-type and mutant Hsp21 were oxidized at a concentration of 0.4 mg protein/mL for 2 h at 37°C with 5.0 mM H2O2 in the different buffer/salt combinations indicated in the figure legends. Oxidation was stopped by the addition of 20 mM DTT. Heat treatment was performed by incubation at 70°C or 90°C for 1 h.
Electrophoresis
Nondenaturing PAGE (3 %–27% gradient of acrylamide) was run at 100 V for 0.5 h and 150 V overnight at 15°C. Nondenaturing PAGE samples were precipitated with acetone (200 µL sample to 1 mL freeze-cold acetone) and resuspended in sample buffer (0.25 M Tris/HCl, 40% [v/v] glycerol) at pH 7.8. Then, 20 µg protein/lane was loaded on the gel. Proteins were detected by Coomassie Brilliant Blue staining. Urea gradient electrophoresis was performed as described previously (Härndahl et al. 1998). Briefly, a horizontal urea concentration gradient (0–8M) was casted perpendicular to the direction of migration, superimposed on a horizontal 15%–11% polyacrylamide gradient to compensate for the urea effect on the electrophoretic behavior of proteins that does not involve unfolding. On the top surface of the gel, 200 µg of pea (P. sativum) Hsp21 was loaded, and electrophoresis was run in a Tris–borate buffer system (pH 8.6 without SDS at 20°C) for 20 min at 10 mA and then for 3 h at 20 mA.
CD and fluorescence spectroscopy
Oxidation-induced changes in the CD spectra of wild-type and mutant Hsp21 protein were recorded as described previously (Härndahl et al. 2001). Conformational stability of wild-type and mutant Hsp21 protein was determined by recording tryptophan fluorescence spectra in increasing urea concentration, using a FluoroMax-2 fluorimeter (Instruments SA) with an excitation wavelength of 295 nm, an excitation slit width of 3 nm, and an emission slit width of 3 nm. Spectra were collected in a 10-mm pathlength quartz cell (Hellma). The sample buffer consisted of 10 mM potassium phosphate at pH 7.0, and protein concentration was 0.05 mg/mL.
Light-scattering assays for chaperone-like activity assays
Thermal aggregation of citrate synthase was induced by incubation at 43°C, and aggregation of insulin was induced by the addition of DTT as previously described (Härndahl et al. 2001). The ability of Hsp21 and the Hsp21 mutants to suppress aggregation was recorded by light scattering measurements.
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