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1 School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom
2 Division of Structural Biology, University of Oxford, Oxford OX3 7BN, United Kingdom
3 Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, United Kingdom
Reprint requests to: Alastair R. Hawkins, School of Cell and Molecular Biosciences, Catherine Cookson Building, Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK; e-mail: a.r.hawkins{at}ncl.ac.uk; fax: +44-191-2227424.
(RECEIVED June 29, 2004; FINAL REVISION August 18, 2004; ACCEPTED August 21, 2004)
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Keywords: NmrA; site-directed mutagenesis; biocalorimetry; nitrogen metabolite repression
Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.04958904.
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Introduction |
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Two major classes of mutant affecting nitrogen metabolite repression have been described. The first class, exemplified by the nmr-1 and nmrA genes of N. crassa and A. nidulans, respectively, has a partially derepressed phenotype (Dunn-Coleman et al. 1979; Andrianopoulos et al. 1998), implying that they act as negative transcription regulators. The second class is represented by the nit-2 and areA genes of N. crassa and A. nidulans, respectively, and they encode the GATA-binding proteins NIT2 and AreA. These proteins contain single zinc fingers and are required for stimulation of transcription of genes controlled by nitrogen metabolite repression (Grove and Marzluf 1981; Davis and Hynes 1987; Fu and Marzluf 1990; Marzluf 1997; Rutter et al. 2001). Loss-of-function mutants are unable to use non-preferred nitrogen sources, and are said to have a repressed phenotype, in contrast to the wild-type repressible phenotype.
We previously reported the structures of the unliganded wild-type form of the nmrA-encoded NmrA protein, as well as complexes with NAD+ and NADP+ (Nichols et al. 2001; Stammers et al. 2001). Structural comparisons revealed that NmrA has an unexpected similarity to the short-chain dehydrogenase-reductase (SDR) superfamily (Stammers et al. 2001), with the closest relationship to UDP-galactose-4-epimerase (UDP-GE). Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) experiments showed that NmrA binds NAD+ and NADP+ with similar affinity but has a greatly reduced affinity for NADH and NADPH. The structure of NmrA in a complex with NADP+ has shown how repositioning a His 37 side-chain allows the different conformations of NAD+ and NADP+ to be accommodated (Lamb et al. 2003). The ability of NmrA to discriminate between the oxidized and reduced forms of the dinucleotides may be linked to a possible role in redox sensing. ITC has demonstrated that NmrA and a C-terminal fragment of the GATA transcription factor AreA interact with a 1:1 stoichiometry and an apparent KD of 0.26 µM. The presence of NAD(P)+ does not significantly affect the strength of the interaction between NmrA and the C-terminal fragment of AreA; moreover, ITC, DSC, and circular dichroism (CD) experiments were unable to find any evidence that NmrA could bind the nitrogen metabolite repression-signaling molecules ammonium or glutamine (Lamb et al. 2003).
The molecular mechanism of the signal transduction pathway responsible for nitrogen metabolite repression in A. nidulans includes control of mRNA stability mediated through the 3' untranslated region of the areA mRNA and AreA-dependent remodeling of chromatin domains (Barredo et al. 1989; Platt et al. 1996; Muro-Pastor et al. 1999). In vitro, the nmr1-encoded N-terminally deleted forms of NMR1 protein bind directly to the zinc finger region and the extreme C terminus of NIT2 (DeBusk and Ogilvie 1984; Young and Marzluf 1991; Xiao et al. 1995; Muro-Pastor et al. 1999). The interactions were demonstrated using the yeast two-hybrid system, EMSA, and column binding techniques using 6-His- and GST-tagged fragments of the NIT2 protein as bait. These latter observations have formed the experimental basis for a model in which NMR1/NmrA are proposed to exert their transcription-repressing affect by inhibiting NIT2/AreA binding to DNA in the presence of free glutamine in the cell (Xiao et al. 1995; Muro-Pastor et al. 1999).
Here we report that (1) a C-terminal fragment of AreA can bind individually to GATA-containing DNA and NmrA, and (2) that in the presence of a mixture of GATA-containing DNA and NmrA, the AreA fragment binds preferentially to the GATA-containing DNA in vitro. In order to further understand the putative redox sensing ability of NmrA, we report the characterization of four mutant NmrA proteins that are either up- or down-modulated in their ability to bind NAD+ without compromising their ability to bind a C-terminal fragment of AreA in vitro. We also report that in contrast to previous predictions, deletion of the final nine C-terminal amino acids of a C-terminal fragment of AreA does not reduce the affinity for NmrA. However, some mutations in the C-terminal domain of NmrA do reduce the affinity of the interaction between NmrA and a C-terminal fragment of AreA.
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We used a fluorescently labeled double-stranded (ds) oligonucleotide containing GATA site 5 as the target DNA in an FP assay (described in Materials and Methods). The FP experiments showed that AreA662 interacted with the fluorescently labeled oligonucleotide and that adding back nonfluorescently labeled DNA containing GATA site 5 competes out this binding (Fig. 1A,B
). In order to confirm whether the interaction between the C-terminal fragment of AreA and DNA is GATA-dependent, we used a mutant ds-oligonucleotide of the same overall nucleotide composition but with the GATA site 5 changed to GTAA, in competition experiments (Fig. 1B). Figure 1B shows that 2.9 µM GATA-containing DNA was able to compete off ~95% of the fluorescently labeled DNA, whereas at a concentration of 5 µM the non-GATA-containing DNA had only competed off ~40%. These data demonstrate that the interaction of AreA662 with DNA is significantly GATA-dependent. The data shown in Figure 1B do not fit to a simple 1:1 binding model, and therefore we were unable to determine the binding constants. Equivalent ITC experiments (see below and Fig. 2A) confirmed that the binding of AreA662 with DNA did not fit to a simple 1:1 binding model.
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, where it can be seen that concentrations of NmrA up to 5.9 µM (~23x the KD for the AreA662:NmrA interaction) the anisotropy signal was reduced by ~24%. The finding that the anisotropy signal was reduced rather than increased indicates that under these conditions the NmrA did not bind to the complex of AreA662/GATA site 5 DNA. Furthermore, the modest reduction in the anisotropy signal (24%) shows that under these conditions the majority of AreA662 remained in a complex with GATA site 5-containing DNA. Similar results were seen when the titrations were then repeated in the presence of a final concentration of 1 mM NAD+ to see whether the oxidized nucleotide affected the binding of AreA662 to NmrA in the presence of the GATA-containing DNA. These results contrast with the data reported for the NMR1/NIT2 proteins of N. crassa (Xiao et al. 1995); therefore, we checked the finding by using an independent assay based on the technique of ITC.
In separate experiments with the same stock of AreA662, we titrated a ds-oligonucleotide containing either GATA site 9 or site 5 into a fixed concentration of AreA until the reaction had gone to completion, thereby capturing the AreA662 in a complex with the GATA-containing ds-oligonucleotide (Fig. 2A). The data in Figure 2A were generated using GATA site 9 and do not fit a simple 1:1 binding model, and therefore they independently verify the observation from the FP assay described above. Similar data were observed when GATA site 5 was used. NmrA was then titrated onto this mixture but caused no additional heat exchange above background, indicating that NmrA was not binding to AreA in the presence of the GATA-containing ds-oligonucleotide (Fig. 2B). We repeated these experiments using a mutant oligonucleotide that has the same base composition as GATA site 5, but has the GATA site changed to GTAA (Table 1). Figure 2C shows that this oligonucleotide when titrated into AreA662 showed a complex and much smaller heat exchange compared to Figure 2A, consistent with weak nonspecific binding. When NmrA was titrated into this mixture, the increased heat exchange shown in Figure 2D was seen. It is possible that the weak nonspecific binding to AreA662 revealed by the use of this non-GATA-containing ds-oligonucleotide is responsible for the inability to fit the data shown in Figures 1B and 2A to a simple 1:1 binding model.
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). A ds-oligonucleotide containing GATA site 9 was then titrated onto this preformed AreA662/NmrA complex, and the resulting heat exchange is shown in Figure 2E. As a control (Fig. 2F), we titrated GATA site 9 onto NmrA and found a minimal heat exchange above the background heat of dilution, indicating that NmrA was unable to bind this DNA, or if it does bind, it does so with zero heat (
H=0). As a positive control to check that the stocks of NmrA and AreA662 used still interacted as previously published (KD 3 0.26 µM), we titrated NmrA onto AreA662 and determined the KD for the interaction as 0.25 µM (see Tables 3,4).
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show that NmrA does not bind appreciably to a GATA-containing ds-oligonucleotide; therefore the heat exchange seen in Figure 2D is most simply interpreted as arising from the binding of NmrA to the free fraction of AreA662 present in the mixture. As the amount of free AreA662 in this experiment is not known, it is not possible to determine the KD for the binding of NmrA to AreA662 in this experiment. The heat exchange seen in Figure 2E is most simply interpreted as meaning that DNA containing GATA site 9 is able to bind to AreA662 even when the AreA662 is precomplexed with NmrA. Because Figures 1C and 2B show that NmrA does not bind to a preformed AreA/GATA ds-oligonucleotide complex under the conditions used, the data imply that in the presence of increasing concentrations of GATA-containing ds-oligonucleotide, NmrA dissociates from AreA662.
Overall, the data shown in Figure 2A–F are most simply interpreted as meaning that AreA662 can bind individually to GATA-containing DNA and NmrA and that in the presence of a mixture of GATA-containing DNA and NmrA, AreA662 binds preferentially to the GATA-containing DNA. As the FP and ITC experiments were carried out in the same buffer, they are directly comparable and independently verify the conclusions drawn.
The extreme C-terminal nine amino acids of AreA662 are not required for the binding of NmrA
The C-terminal nine amino acids of AreA and its homologs (including NIT2 in N. crassa) are highly conserved across a range of filamentous fungi, and deletion mutants of areA lacking these residues have a partially derepressed phenotype (Platt et al. 1996). The yeast two-hybrid system was used to study the interaction of the C-terminal 223 residues of NIT2 with a GAL4 DNA-binding domain fused to the NMR protein. The NIT2 and NMR proteins were reported to interact in this system, and mutations that deleted or caused point mutations in the C-terminal 12 amino acids of NIT2 were reported to disrupt this interaction (Pan et al. 1997). When equivalent C-terminal mutations were made in the wild-type nit-2 gene, the mutations caused a substantial loss of nitrogen repression in vivo (Pan et al. 1997). Given these observations, we wished to test the hypothesis that the C-terminal nine amino acids of AreA662 are required for NmrA to bind.
We constructed a mutant version of AreA662 lacking the C-terminal nine amino acids, and designated the mutant protein AreA6629 (Table 1). The mutant protein was purified in bulk and used in ITC experiments with NmrA, where it was found that the two proteins interacted with a 1:1 stoichiometry and KD (0.26 3M) indistinguishable from that of the interaction between NmrA and AreA662 (Table 4). Purified AreA6629 was also used in FP experiments with a HEX-labeled ds-oligonucleotide containing GATA site 5 (Table 1), and was found to bind in a similar manner to AreA662 (data not shown).
Site-directed mutagenesis can up- and down-modulate dinucleotide binding to NmrA
NmrA lacks the characteristic nucleotide binding motif GXXGXXG found in related SDRs such as UDP galactose epimerase (Thoden et al. 2001), having instead the sequence NXXGXXA. The crystal structures of NmrA in complexes with NAD+ and NADP+ have highlighted residues T14 and H37 as being important in the interactions with the AMP portion of NAD+. In particular, H37 ring stacks with the adenosine base of both NAD+ and NADP+ and undergoes a flip between the two complexes, and a change of H37 to W37 is predicted to result in tighter binding of NAD+. To test this hypothesis and see whether it is possible to modulate nucleotide binding without compromising AreA662 binding, we made three mutations in the Rossman fold and three in the C-terminal domain of NmrA. Table 1 summarizes the details of the mutants constructed. The dinucleotide binding properties of the various mutant proteins were compared to the wild-type protein by DSC analysis or in one case by ITC analysis, and AreA662 binding properties were analyzed by ITC. Although the DSC unfolding transitions are irreversible under the conditions used, the ratio of calorimetric to van’t Hoff enthalpies is close to unity (Table 3) and is consistent with cooperative unfolding of a monomeric protein unit. The properties of the mutant proteins are summarized in Tables 3 and 4, where it can be seen that all of the mutant proteins retained the ability to bind to AreA662 but three had altered affinities for dinucleotides compared to the wild-type.
The mutant proteins N12G/A18G and T14V have diminished affinity for dinucleotides, whereas the H37W mutant has an ~4.2-fold increase in affinity for NAD+ and essentially wild-type affinity for NADP+. This estimate of NAD affinity for the H37W mutant was measured by ITC and is a direct measure of the interaction under the buffer and temperature (25°C) conditions chosen. DSC, on the other hand, is an indirect measure of binding at an elevated temperature, and the values quoted in Table 3 derived by this technique give only a general guide to the extent of the changes in affinity for the various ligands shown by each mutant protein. ITC cannot be used to estimate the KD values of NmrA mutants with reduced affinity for oxidized nicotinamide dinucleotides compared to wild-type, as the affinity is too low to be measured by this technique. For the same reason ITC cannot be used to measure the binding of reduced dinucleotides to either wild-type or mutant NmrA. However, the protein that has a control substitution in the C-terminal domain (E263Q/E266Q) has DSC-estimated KD values for oxidized nucleotide binding that differ from the wild-type value by ~25%. This reasonable match between affinities of the C-terminal mutant and the wild-type gives confidence that the much larger apparent differences between the Rossman fold mutants and wild-type do reflect specific changes in the affinity of nucleotide binding. The DSC-estimated KD values for the binding of wild-type NmrA to NAD+ and NADP+ reported here differ by ~27% and 2.8-fold, respectively, from those previously published (Lamb et al. 2003). The differences may be due to the fact that the KD values reported here were determined in 50 mM potassium phosphate, pH 7.2, whereas the previously published values used potassium phosphate buffer at pH 6.6.
Structure of the NmrA N12G/A18G mutant protein provides a molecular explanation for the reduction in affinity for dinucleotides
As the GXXGXXG or closely related motifs are present in many Rossman fold-containing proteins, it was surprising that engineering in this sequence into NmrA resulted in a diminution of the ability to bind oxidized dinucleotides. In order to try and understand the molecular basis for this reduced affinity for oxidized dinucleotides, we determined the crystal structure of the N12G/A18G double mutant NmrA to 1.4 Å resolution. The statistics for crystallographic structure determination are shown in Table 5. The electron density map for NmrA N12G/A18G confirmed the presence of the mutations at N12G and A18G. Comparing the refined model of NmrA N12G/A18G with either unliganded NmrA or in a complex with NAD shows significant changes in main chain position in the region of the N12G mutation (Fig. 3). The CA atom of N12G is displaced by 1.9 Å compared to wild-type against an overall rmsd of 0.26 Å for all CA atoms. There is also a loss of a hydrogen-bond from the N12 amide group to His37; the latter side chain then rotates around 
1 (CA-CB atoms) away from its position in both the wild-type unliganded and NAD+-bound NmrA structures toward the conformation found in the complex of NmrA with NADP+ (Stammers et al. 2001). The movement of N12G main chain is a direct result of the A18G mutation, because there is a van der Waals contact between the A18 side chain and the carbonyl oxygen of N12 in wild-type NmrA. In NmrA N12G/A18G, the smaller bulk of G18 allows movement of the main chain in this region such that a contact with residue 18 (via the CA atom) is maintained. N12 forms van der Waals contacts with the adenine ring of NAD+ that are lost directly as a result of N12G or indirectly by the A18G mutation. Such changes together with the repositioning of H37 appear able to account for the reduced binding for NAD+. The different H37 position means that the N12-H37 hydrogen bond does not need to be broken to be correctly orientated for binding NADP+; hence this may explain the relatively lower loss of affinity for this dinucleotide to the N12G/A18G NmrA.
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6 forms part of the C-terminal domain, is slightly recessed but is solvent-accessible, and has a particularly long cluster of conserved residues (Stammers et al. 2001). For this reason it was suggested that the C-terminal domain of NmrA is involved in binding to AreA and that helix 6 could be directly involved in the interaction (Stammers et al. 2001). To test this hypothesis directly we made two double mutations in this helix, E193Q/D195N and Q202E/F204Y (Table 1) and compared their properties to the doubly mutant E263Q/ E266Q protein that has the mutant substitutions in helix 9. Using ITC and DSC, we tested the ability of the purified mutant proteins to bind AreA662 and dinucleotides (see Tables 3 and 4). In each case, the mutant proteins were not compromised in their ability to bind dinucleotides, and mutant Q202E/F204Y had an ~2.5-fold increase in KD for AreA662. The E193Q/D195N protein lost its ability to bind AreA662 when stored on ice for 10 d; however, the ability to bind oxidized dinucleotides was unaffected (Tables 3,4) during this time period. The ability of the Q202E/F204Y protein to bind AreA662 and oxidized dinucleotides was unaffected over this time period. The E263Q/E266Q protein was undiminished in its affinity for AreA662 and oxidized dinucleotides over the 10-d period. |
Discussion |
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We show here for the first time that, in vitro, a C-terminal fragment of the AreA protein has its affinity for native NmrA protein greatly reduced in the presence of GATA-containing DNA. These observations are consistent with a model in which NmrA and GATA-containing DNA compete for a single or two overlapping sites in the C-terminal region of AreA (Xiao et al. 1995). This model predicts that at high molar excess NmrA should show some competition with GATA-containing DNA for the common or overlapping site on AreA. The data shown in Figure 1C are consistent with this prediction. These data contrast with the report that the homologous NMR1 protein of N. crassa inhibits the binding of NIT2 to DNA. In those experiments, the NMR1 protein was deleted for the N-terminal 46 amino acids, and the NIT2 fragment was that encoded by the nit-2 KpnI-EcoRI sequence that specifies ~326 amino acids including the zinc finger DNA-binding motif (Xiao et al. 1995). Both the NMR1 and NIT2 fragments used were GST-fusions, and it has been shown that the GST moiety of fusion proteins can dimerize (Panayotou et al. 1993; Ladbury et al. 1995). Interpretation of the reported data is therefore complicated, because any association/dissociation of the NIT2 and NMR1 fragments and their interaction with DNA will be affected by a contribution from the dimerization of the associated GST moieties. The proteins we report here are native NmrA and an N-terminally truncated AreA that contains a small (41 amino acids) pRSETb-encoded fusion sequence containing six His residues. As we previously reported, NmrA binds to C-terminal fragments of AreA and does not bind to the pRSETb-encoded N-terminal fusion sequence (Lamb et al. 2003). Therefore, the analysis of the interactions between NmrA and C-terminal AreA fragments reported here is not complicated by the potential interactions between other sequences fused to NmrA or AreA fragments.
Our results further contrast with data reported for the NIT2:NMR interaction in that we found that the C-terminal nine amino acids of AreA662 are not required for the binding of NmrA. In the N. crassa study, loss of binding between NIT2 and NMR in the yeast two-hybrid system by deleting or mutating the C-terminal 12 amino acids was correlated with a substantial loss of nitrogen repression by the equivalent mutation in vivo (Pan et al. 1997). This phenotype is similar to the partial derepression reported for areA mutants lacking the C-terminal nine amino acids. It is possible that these contrasts may be highlighting some differences in the detailed mechanism of nitrogen metabolite repression between N. crassa and A. nidulans. Alternatively, with hindsight the use of the two-hybrid system may not be optimal to study this particular interaction, as cases of false positives and false negatives have been reported and discussed (Legrain et al. 2001).
The data we present here suggest that, in vivo, NmrA does not prevent the binding of full-length AreA to its cognate promoters under conditions of nitrogen metabolite repression. This suggestion is supported by the observation that in vivo GATA site 5 is occupied by AreA even under conditions of nitrogen metabolite repression in a strain with a wild-type nmrA gene (Muro-Pastor et al. 1999). These observations imply that in vivo NmrA exerts its repressing effect on transcription by an indirect route, possibly by controlling the rate of entry of AreA into the nucleus. As NmrA can discriminate between oxidized and reduced dinucleotides (Lamb et al. 2003), it is possible that NmrA may influence the rate of entry of AreA into the nucleus in response to the redox state of the cytoplasm.
There is a precedent in Saccharomyces cerevisiae for the regulation of the use of different nitrogen sources by the cytoplasmic retention of a GATA-binding transcription activating protein. S. cerevisiae has a preferred nitrogen source (glutamine) and uses this selectively by repressing GATA factor-dependent transcription of the genes needed to transport and catabolize nonpreferred sources, in a physiological process called nitrogen catabolite repression (Cox et al. 2000). In this system, GATA-dependent transcription is stimulated by the activator proteins Gln3p and Gat1p. When nitrogen is limiting, Gln3p and Gat1p are found primarily in the nucleus, but when nitrogen is in excess they are excluded from the nucleus. Nuclear exclusion of Gln3p is proposed to involve the formation of a complex between Gln3p and the NCR regulator Ure2p (Beck and Hall 1999; Cox et al. 2000; Kulkarni et al. 2001). The interaction between Gln3p and Ure2p shows an interesting parallel with the AreA/NmrA interaction, and our data suggest that control of access of nutrient-regulated transcription factors to the nucleus by cytoplasmic proteins may be a more widespread mechanism than previously realized.
The program of site-directed mutagenesis we report here shows that it is possible to up- and down-modulate the affinity of NmrA for oxidized dinucleotides without affecting the ability of the mutant NmrA to bind to AreA662. Similarly, it proved possible to reduce the affinity of NmrA for AreA662 without significantly reducing the ability of the mutant proteins to bind oxidized dinucleotides. Surprisingly, the introduction of the classic GXXGXXG motif into the Rossman fold of NmrA caused a diminution rather than increase in affinity for the oxidized dinucleotides. However, the molecular basis for this observation is explained by the structure of the N12G/A18G mutant protein, which shows significant changes in main chain position in the region of the N12G mutation. The change in main chain position and repositioning of the H37 side chain disrupts van der Waals/ aromatic ring stacking contacts with the adenine ring of NAD+, thus causing a reduction in binding for this nicotinamide dinucleotide.
The fact that NmrA has different sequence requirements for binding NAD+ compared to structurally related SDRs may be significant. Clearly it is less likely that NAD+ binding in NmrA is a vestigial property left over from its proposed evolution from a dehydrogenase enzyme, because the "original" SDR-like sequence binds NAD+ less tightly. Indeed, it is consistent with the view that NAD+ binding in NmrA relates to a distinctive biological role such as a redox-sensing control property rather than for a catalytic function, as is the case in SDRs. In contrast to the weakened NAD+ binding of the N12G/A18G NmrA mutant, we show that it also possible to generate a mutant with increased binding for NAD+ viz. H37W.
Helix 6 double mutant Q202E/F204Y has a modest but significant increase in the KD for the binding of NmrA and AreA662 without increasing the KD for oxidized dinucleotide binding (see Tables 3,4), and this result is consistent with helix 6 being directly involved in binding to AreA. The fact that a control mutation, E263Q/E266Q, in helix 9 does not impair AreA662 or oxidized nucleotide binding lends confidence to this interpretation. Interestingly, in DSC analysis the thermal transition midpoint temperatures (Tm) for the unliganded doubly mutant protein E193Q/D195N is dramatically lower (~10°C) than the wild-type protein (Table 3). These mutant substitutions do not increase the Km for dinucleotide binding but do have a large negative effect on AreA662 binding that increases with time. These observations imply that in the doubly mutant E193Q/D195N protein, the integrity of the Rossman fold is not compromised, and that a large effect of the mutant substitutions is on the stability of the C-terminal domain of NmrA (Stammers et al. 2001). These observations argue strongly that the C-terminal domain of NmrA is responsible for the interaction with AreA662, and that the two domains of NmrA function independently.
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Molecular biology and biochemistry
Routine molecular biology protocols followed individual manufacturer’s recommendations or were as described (Maniatis et al. 1982; Lamb et al. 1996). All DNA sequencing was carried out on double-stranded plasmid DNA using an ABI PRISM 377 DNA sequencer in the University of Newcastle upon Tyne Facility for Molecular Biology.
Site-directed mutagenesis
Site-directed mutagenesis was as described (Hemsley et al. 1989) using the oligonucleotides shown in Table 1. The plasmid substrate for areA mutagenesis was pRF48 and for nmrA was pTR121 (Nichols et al. 2001; Lamb et al. 2003).
Purification of NmrA and a C-terminal fragment of AreA
NmrA wild-type and mutant, and C-terminal fragments of AreA (designated AreA662 and AreA6629) were purified using the protocols described (Nichols et al. 2001; Lamb et al. 2003).
Protein and DNA concentrations
Protein and DNA concentrations were measured spectrophotometrically as described (Gill and von Hippel 1989) using the known (DNA) or theoretical molar absorption coefficient calculated by the Vector Nti suite of programs (protein).
Isothermal titration calorimetry
Isothermal titration calorimetry experiments at 25°C were performed using a high-precision VP-ITC titration calorimetric system (Microcal). For the study of protein:protein interactions, NmrA in the injection syringe was titrated into AreA662 or AreA6629, in the calorimetric cell (1.4 mL) in the concentration ranges described in the legends to Tables 2 and 4. For the study of protein:DNA interactions, the ds-oligonucleotides in the injection syringe were titrated into AreA662 in the calorimetric cells. For a description of the more complex titrations, see Table 2. The proteins were dialysed into 50 mM potassium phosphate pH 7.2, 1 mM DTT, and the DNA was dissolved in the same buffer. Oligonucleotides 522 and 523 were annealed to produce GATA site 5, and oligonucleotides 524 and 525 were annealed to produce a mutant GATA site 5 in which the GATA motif was changed to GTAA. Oligonucleotides 528 and 529 were annealed to produce GATA site 9. Oligonucleotides were annealed as described (Reid et al. 2001). The heat evolved following each 10-µL injection was obtained from the integral of the calorimetric signal. The heat due to the binding reaction was obtained as the difference between the heat of reaction and the corresponding heat of dilution. Analysis of data was performed using Microcal Origin Software.
Differential scanning calorimetry
Differential scanning calorimetry measurements on NmrA and NmrA mutants were made using a Microcal VP-DSC instrument at a scan rate of 1°C per min and a filtering period of 16 sec. Proteins were dialysed in 50 mM potassium phosphate, pH 7.2, 1 mM DTT, and the dialysis buffer was retained to dissolve ligands, to dilute proteins, and for baseline controls. The concentrations of protein and ligands are given in the Table 3 legend. Ligand-induced shifts in thermal transition midpoint temperatures (Tm) were used to estimate approximate ligand affinities using standard thermodynamic methods as described (Cooper 1999; Cooper et al. 2000).
Fluorescence anisotropy assays
Oligonucleotides 519 and 522 were annealed to produce GATA site 5 with a Hexachlorofluorescein (HEX) label. Fluorescence anisotropy measurements were carried out at 25°C using an SLM-Aminco 8100 fluorimeter in a buffer consisting of 50 mM potassium phosphate, pH 7.2, 1 mM DTT. The excitation wavelength was 530 nm (slit width 8 nm), and the emission was detected through a 3-mm thick 570-nm cut-off filter. A 1-mL fluorescence cuvette with excitation and emission pathlengths 10 mm each was used. For measurements for the direct binding of AreA662 or AreA6629 to GATA-containing DNA, the initial volume was 1 mL, and small volumes of AreA662 or AreA6629 were added to the fluorescent oligonucleotide; the anisotropy was measured after each addition. For competition titrations, small volumes of oligonucleotide were added to a preformed AreA662/Hex-labeled GATA site 5 complex, and the anisotropy was measured after each addition. Anisotropy was measured as described (Reid et al. 2001).
Protein crystallization and structure determination
Unliganded NmrA N12G/A18G was crystallized in the trigonal crystal form as described for wild-type protein (Nichols et al. 2001; Stammers et al. 2001). Crystals were flash frozen using 20% glycerol as cryoprotectant and maintained at 100 K in a stream of cooled nitrogen gas. X-ray diffraction data for NmrA N12G/A18G crystals were collected to 1.4 Å resolution at the European Synchrotron Radiation Facility beam-line ID14EH4 (
= 0.9323 Å) equipped with an ADSC Quantum 4 CCD detector. A total of 180 data frames of 1° oscillation were collected, giving 69,599 unique reflections with ninefold redundancy for 25.0–1.40 Å resolution. The data frames were indexed and integrated with DENZO and scaled with SCALEPACK (Otwinowski and Minor 1996).
The NmrA N12G/A18G structure was solved by rigid-body refinement using the CNS program with the wild-type NmrA coordinates (PDB code 1K6I
[PDB]
) as the starting model. The NmrA model was rebuilt from 2Fo-Fc and Fo-Fc maps using the O program on a Silicon Graphics Octane workstation, and the N12G/A18G mutations incorporated. Rounds of positional and B-factor refinement with anisotropic B-factor scaling and solvent correction were carried out using CNS. The refined model contains 325 protein residues, 606 water molecules, one glycerol molecule, one phosphate, four potassium and five chlorine ions. There is no density for residues 1–2 and the flexible glycine-rich loop 284–308, which are not modeled in the structure. The electron density shows clearly double conformations for the side chains of residues Asn80, Thr82, Arg239, and Arg329. The final model has a working R-factor of 0.193 (Rfree = 0.238) for data in the resolution range 25.0–1.40 Å, with the retention of good stereochemistry (Table 5).
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Acknowledgments |
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References |
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