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Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
Reprint requests to: Zheng-yu Peng, MC3305, Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA; e-mail: peng{at}nso2.uchc.edu; fax: (860) 679-3408.
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Abstract |
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 TOP Abstract IntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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Keywords: ankyrin repeat; repeat motif; p16; protei–protein interactions
1 Present address: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. 
Article and publication are at http://www.proteinscience.org/cgi/doi/10.1110/ps.03554604.
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Introduction |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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The ankyrin repeat, a 33-residue sequence motif, was first identified in the yeast cell cycle regulator Swi6/Cdc10 and the Drosophila signaling protein Notch (Breeden and Nasmyth 1987), and was eventually named for the cytoskeletal protein ankyrin, which contains 24 copies of this repeat (Lux et al. 1990). Subsequently, ankyrin repeats have been found in many proteins spanning a wide range of functions. The hand curated database PFAM-A, as of October 2003, contains 9689 ankyrin repeat sequences in 1871 proteins identified from the Swissprot and SP-TrEMBL databases (Bateman et al. 2002), while the SMART database contains 19,276 ankyrin repeat sequences in 3608 proteins identified from the nonredundant protein database (NRDB; Schultz et al. 1998; Letunic et al. 2002). Ankyrin repeat proteins are present in all three superkingdoms including bacteria, archaea, and eukarya, as well as in a number of viral genomes. However, a phylogenetic breakdown of the organisms that contain ankyrin repeats indicates that the majority are found in eukaryotes. Modular protein domains, such as the ankyrin repeat, that act as a scaffold for molecular interactions are likely important for development of the numerous signaling pathways necessary to evolve a more complicated multicellular organism (Marcotte et al. 1999). Some ankyrin repeat proteins have been found in viruses and bacteria, but these are believed to be the result of horizontal gene transfer (Bork 1993).
The number of ankyrin repeats found in a single protein varies greatly. Analysis of the SMART and PFAM databases show that the number of repeats per protein reaches from one to 33, with the majority of proteins containing six or fewer repeats (Fig. 1
). The protein with the most ankyrin repeats to date is the ORF EAA39756
[GenBank]
from Giardia lamblia, which actually may contain 34 repeats if the PFAM and SMART predictions are combined. Analysis of the SMART database suggests that two repeats are the most commonly occurring number of repeats per protein, while data from the PFAM database suggest three as the most popular number of repeats. Detection of the terminal repeats in an ankyrin repeat domain is difficult due to the divergence of many well-conserved hydrophobic residues. Through evolution, these positions have been replaced with polar residues to facilitate favorable interactions with the solvent, and thus the terminal repeats often deviate from the established consensus sequence. Also, the terminal repeats are frequently truncated, and therefore, the search model may not identify these sequences. Given this limitation, the most popular number of repeats per protein may actually be one or two repeats greater than that shown in Figure 1. For example, although Figure 1 shows that a significant number of proteins contain a single ankyrin repeat, we have previously demonstrated that an isolated ankyrin repeat cannot adopt a folded structure unless it shares an interface with another repeat (Zhang and Peng 2000; Mosavi et al. 2002a).
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). Ankyrin repeats have been observed to exist by themselves as a single domain protein or in conjunction with other domains in the same protein. Each repeat folds into two antiparallel 
-helices followed by a 
-hairpin or a long loop. Consecutive repeats stack together to form an L-shaped domain, which resembles a cupped hand with the -hairpins representing the fingers and the helices as the palm. The overall shape of a typical ankyrin repeat domain is slightly curved, and this feature is particularly apparent in the structure of proteins that contain a large number of repeats (e.g., Fig. 2h).
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B, a transcription factor that regulates inflammatory response is inhibited by IB, which contains seven ankyrin repeats. Various numbers of ankyrin repeats are found in members of the TRP cation channel family, which include heat and cold sensitive receptors as well as mechanosensory proteins. |
Structural features of the ankyrin repeat |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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-hairpin/loop fold. The helices are arranged in anti-parallel fashion followed by a loop region that points outward at an approximately 90° angle and forms a -hairpin in some known ankyrin repeat structures. The helices of one repeat pack against the helices of the adjacent repeat while the -hairpin/loop region, in some cases, forms a continuous -sheet. The interrepeat interface mainly consists of hydrophobic interactions stabilizing the helices in addition to a hydrogen bonding network connecting the -hairpin/ loop region. The outer helix is nine residues long, spanning from positions 15–24 of the canonical sequence, while the inner helix is seven residues long and extends from positions 5–12 (Fig. 3A). The overall stack exhibits a slight curvature resulting from the difference in helix length as well as the interrepeat packing interactions between the two helices. Near the short loop of the helix-loop-helix, the presence of a small side-chain residue at position 10 allows the shorter inner helices to pack more closely against each other while the outer helices are spaced further apart due to midsize residues at positions 17 and 18. Closer to the -hairpin/loop junction, positions 6, 21, and 22 typically contain residues with large side chains, such as leucine, which lends the ankyrin repeat stack a left-handed superhelical twist. Each repeat is rotated 2–3° counterclockwise relative to the preceding repeat and contributes approximately 13° of pitch to the superhelical turn of the molecule (Michaely et al. 2002). A complete turn of the superhelix would require 32 repeats and have a 35 Å radius (Michel et al. 2001).
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-hairpin/loop. In addition, well-conserved glycines at positions 13 and 25 contribute to helix termination thereby fostering the transition into a tight loop between the helices and the beginning of the long -hairpin/loop region, respectively. The helical packing interactions of neighboring ankyrin repeats are essentially identical to that of a four-helix bundle. The nonpolar residues at positions 9 and 18 (intra-repeat), positions 8, 10, 17, 20, and 22 (interrepeat) and positions 6 and 21 (both) form the hydrophobic core. For the most part, these positions have relatively low solvent accessibility (Fig. 3C
). However, in terminal repeats that are exposed to solvent, these positions are often replaced by polar residues.
For motifs that have a well-conserved structure but many different functions, one might expect the surface residues to exhibit a high degree of variability to accommodate assorted binding interactions. In the ankyrin repeat, the information content or conservation level of each position does correlate somewhat with solvent accessibility. Previously, we classified each of the 33 positions in the ankyrin motif as either well-conserved, semiconserved of the same type, semiconserved of different type, and nonconserved (Fig. 3A; Mosavi et al. 2002a). As a comparison, we have determined the information content (in bits) of each position in this motif using the currently available repeats downloaded from the PFAM database (http://pfam.wustl.edu/) with the program ALPRO (Schneider and Stephens 1990; Fig. 3B). Generally, the degree of information content calculated by ALPRO agrees well with our original classification scheme, particularly for the well-conserved residues, which display some of the highest values. Minor differences between our classification and the ALPRO calculation exist probably because these analyses treat the amino acid distribution in a different manner. Comparison of the information content to the average solvent accessibility for each of the 33 positions (for internal repeats of identical repeat protein, 4ANK) suggests that most positions with high information content have low solvent accessibility (less than 10%), while the positions previously classified as nonconserved (yellow markers) show high solvent accessibility and low information content (Fig. 3C). Interestingly, a number of the semiconserved residues have both low solvent accessibility and information content.
Covariation or correlation analysis has been be used to expose the distance information and energetic coupling between residues in a large protein family (Gobel et al. 1994; Shindyalov et al. 1994; Lockless and Ranganathan 1999; Larson et al. 2000). Using the ankyrin repeat sequences in the PFAM database, we have performed covariation analysis of all 33 positions in this motif in an effort to reveal positions that may be structurally "coupled" to one another. Several pairs of positions had covariation scores significantly above the mean. The majority of these pairs are located in close proximity to one another (<5 Å) and form interactions in the inter-helical interfaces as well as in the -hairpin/loop region (Fig. 4). Such geometrical complementarities between residues may be important for the stability of the ankyrin repeat.
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-hairpin. For example, both I and Bcl-3 have short insertions between repeats 1 and 2 as well as repeats 3 and 4 in the long loop (Fig. 2d–e). The ankyrin repeat domain of Swi6 contains an insertion between repeats 2 and 3, and another unusually long 40-residue insertion between repeats 3 and 4, both in the long loop (Fig. 2l). Shortening of the helices by deletion of one or two residues has been observed as well. The crystal structures of the INK4 family members p16, p18, and p19, all have a slightly distorted inner helix due to the shortening in the second repeat (Fig. 2a–c). |
Folding and stability of ankyrin repeat proteins |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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The ankyrin repeat domain of Drosophila Notch has been used as a model system to investigate the long-range coupling among repeats (Zweifel and Barrick 2001; Bradley and Barrick 2002). Notch is a signaling molecule that contains seven ankyrin repeats in the intracellular domain. Experiments on the full ankyrin domain (Nank1–7*) as well as two variants with deletion of one or two C-terminal repeats, Nank1–6* and Nank1–5*, respectively showed all proteins to fold in a two-state manner. However, Nank1–7* had significantly higher stability (
GH2O = 8.03 kcal•mole–1) than Nank1–6*(GH2O = 4.14 kcal•mole–1) and Nank1–5*(GH2O = 3.55 kcal•mole–1), indicating that the seventh repeat contributed more to global stability than the sixth repeat (Zweifel and Barrick 2001). These results suggest that although each repeat has some level of intrinsic stability, the effects are felt "globally" over the entire protein. In an effort to uncouple the Notch ankyrin repeats, Nank1–7* constructs were made with a disruptive alanine to glycine substitution in helix 1 of each repeat. Repeat 6 was the only repeat in the Notch ankyrin domain that was severely affected by the substitution and caused the folding of Nank1–7* to go from two-state to multistate. The fact that the long-range cooperativity was interrupted suggests that the interrepeat coupling was weakest through the region of repeat 6 (Bradley and Barrick 2002).
Although the bulk of the equilibrium folding studies on ankyrin repeats strongly suggest that this domain follows a two-state folding pathway, work on human p19 has provided evidence of a partially folded intermediate by NMR studies. Chemical denaturation experiments showed completely superimposable fluorescence and CD chemical denaturation curves. However, a series of 1H-15N HSQC spectra at various urea concentrations revealed the presence of unidentified cross-peaks that were not present in the folded or unfolded states. In addition, urea-induced folding and unfolding transitions monitored by NMR did not intersect at half fraction unfolded, leading to the conclusion that an intermediate with 30% maximum population was present in the equilibrium folding pathway (Zeeb et al. 2002). This intermediate may be an oligomer because of the high concentrations required for NMR studies.
The kinetic folding of ankyrin repeat proteins has been studied for human p16 and p19, both members of the INK4 tumor suppressor family (Tang et al. 1999; Zeeb et al. 2002). Although p16 has four repeats and p19 has five repeats, they share 40% sequence identity and a high degree of structural similarity. Both proteins show three refolding phases at low urea concentrations and one unfolding phase. The slowest phase for both proteins is urea independent and can be accelerated with the addition of a prolyl isomerase such as cyclophilin indicating that proline isomerization is responsible for the rate limiting step. The fast phases result in partial formation of -helical secondary structure, although the details are not well resolved. The kinetic folding pathway of p16 has also been studied using 
-value analysis (Tang et al. 2003). P16 constructs with core mutations designed to subtly decrease the hydrophobic contacts in each repeat were created. Equilibrium folding studies showed a large number of -values close to 1 in the C-terminal two repeats while the -values on the N-terminal two repeats were generally smaller. These results indicate that the C-terminal repeats fold first and may form a "structural scaffold" on which to fold the less stable N-terminal repeats. Indeed, truncation studies of p16 showed that the C-terminal two repeats can form an autonomously folded structure while the N-terminal two repeats are disordered in isolation, suggesting that the two subdomains have very different intrinsic stabilities (Zhang and Peng 2000).
Various truncation studies of ankyrin repeat proteins have been used to investigate the minimum folding unit of this motif. Limited proteolysis combined with deletion studies of ankyrin, which contains 24 repeats, suggested that four to six repeats were necessary to form a stably folded structure, and a single repeat was not capable of folding (Michaely and Bennett 1993). The above-mentioned truncation studies of p16 demonstrated that the C-terminal two repeats could form an autonomously folded unit and suggested that two repeats are actually the minimum folding unit of the ankyrin repeat (Zhang and Peng 2000). Work on our consensus designed ankyrin repeat protein supports this conclusion. 2ANK, with two identical ankyrin repeats, adopted a fully folded conformation after substitution of six surface leucine residues with arginine (Mosavi and Peng 2003).
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Ankyrin repeat-mediated protein–protein interactions |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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. These proteins and their corresponding complexes are essential for many cellular processes such as cell fate determination, endocytosis, transcription regulation, and cell cycle control.
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-hairpin/loop region and inner short helices. Figure 5 illustrates the contact surfaces of four different ankyrin repeat proteins. Four structures of the INK4 family of tumor suppressors in complex with Cdk6 have been solved: two of p19 and one each of p16 and p18. All three proteins have very similar contacts in the interface with Cdk6 mostly involving H-bonds and salt bridges (Fig. 5C). The electrostatic complementarity of the interface is likely responsible for the specificity of p16 and p19 for Cdk4 and Cdk6. Similar to p16 or p19/Cdk6 binding, the interface of the inhibitor IB- with transcription factor NF-B is heavily influenced by contributions from the inner helices (Fig. 5B). In contrast, the structures of the transcription factor complex GABP-/GABP- (Fig. 5A) and the tumor suppressor p53 with regulator 53BP2 (Fig. 5D) show the -hairpin/loops to be the key participant in forming the binding interface. The 53BP2/p53 interface, which also includes a SH3 domain (upper red patch in Fig. 5D), uses only the -hairpin/loop of the fourth ankyrin repeat. GABP-, on the other hand, includes portions of the inner helices in the interface with GABP-.
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Disease-related defects in ankyrin repeat proteins |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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-hairpin/loop region, which may account for the unusually low thermodynamic stability (~3.3 kcal/mole) and high backbone flexibility that leave the protein particularly vulnerable to even subtle mutations (Tevelev et al. 1996).
Naturally occurring mutations in p16 associated with familial melanoma have been well characterized, including R24P, D74N, R87P, P81L, P114L, G101W, and V126D. These seven mutants represent a cross-section of the various defects that interfere with the biological function of this important tumor suppressor molecule. Residues D74 and R87 are located at positions that directly interact with the cyclin-dependent kinases (Russo et al. 1998). These positions are completely conserved among INK4 family members and p16 orthologs. In p16, D74 is located in the loop of repeat 2, while R87 is in the inner helix of repeat 3. These mutations disrupt the electrostatic interactions and hydrogen-bonding network at the binding interface, which in turn, reduce the ligand binding affinity and cell-cycle inhibitory function (Koh et al. 1995; Reymond and Brent 1995; Parry and Peters 1996; Ruas et al. 1999). Indeed, biophysical studies show that D74N retains a native-like structure (Tevelev et al. 1996; Tang et al. 1999). The aspartate to proline substitution at position 87 also effectively "breaks" the -helix, thereby reducing the secondary structure of p16 as indicated by CD studies (Zhang and Peng 1996). The mutation R24P removes electrostatic and spatial constraints located between the -helices of repeat 1, which are important in coordinating the neighboring contact residues R22 and E26. In addition to structural perturbations such as reduced helical content and stability, R24P also forms higher order oligomers, which may reduce kinase binding and cell cycle inhibition (Tang et al. 1999; Walker et al. 1999; Cammett et al. 2003).
In contrast to the above point mutations that introduce proline, P81L and P114L remove the original torsional constraints imposed at these positions. These mutations are located in the highly conserved TPLH motif in the third and fourth repeat, respectively. Both mutants are classified as molten globules based on retention of wild-type–like secondary structure but loss of a cooperative thermal unfolding transition. Additional defects include increased hydrophobic surface exposure and greater susceptibility to proteolysis (Tevelev et al. 1996; Zhang and Peng 1996; Tang et al. 1999; Cammett et al. 2003). Interestingly, P81L remains monomeric while P114L forms higher order oligomers and aggregates. These mutants have greatly reduced affinity for Cdk4 and Cdk6 and are deficient in cell cycle inhibitory function (Lukas et al. 1995; Reymond and Brent 1995; Walker et al. 1999).
Mutations G101W and V126D represent a unique class of temperature sensitive mutations that display diminished biological function as the temperature increases from 30°C to 42°C (Parry and Peters 1996; Ruas et al. 1999). The glycine at position 101 is important for termination of helix-2 in the third repeat. Introduction of the bulky hydrophobic tryptophan side chain disrupts this event at a well-conserved position in the ankyrin consensus (Fig. 3A, position 25). G101W is a molten globule, rapidly proteolyzed and forms large aggregates during sedimentation equilibrium analysis (Zhang and Peng 1996). The side chain of valine, at position 126 in helix-2 of the last repeat, protrudes into the hydrophobic core of repeats 3 and 4. V126D displays reduced secondary structure, increased hydrophobic surface area, and higher order oligomer formation, indicating that substitution with the charged aspartate disrupts the hydrophobic contacts in the C-terminal half of the protein (Tevelev et al. 1996; Zhang and Peng 1996; Tang et al. 1999; Cammett et al. 2003). Aggregation is a process that is accelerated at higher temperature, leading to reduction in the concentration of available p16, which may explain the temperature sensitive phenotype of these mutant proteins (Walker et al. 1999; Becker et al. 2001).
Taken together, these studies illustrate how each mutation contributes to the global destabilization and functional inactivation of p16. Indeed, amino acid substitutions that enhance the stability of p16 without altering its structure or function show increased resistance to the destabilizing effects of different oncogenic mutations, independent of where they occur in the molecule. Recently, a hyperstable variant of wild-type p16 was shown to retain wild type-like Cdk4 binding activity in the presence of the melanoma-associated mutations R24P, P81L, or V126D (Cammett et al. 2003). This apparent global suppressor effect indicates that increasing the thermodynamic stability of p16 could be a general strategy to restore function to defective mutant proteins.
The Notch family of single-pass transmembrane receptors are involved in cell fate determination. The intracellular domain of Notch contains seven ankyrin repeats that may be necessary for recruitment of transcriptional coactivators when the intracellular domain is translocated into the nucleus (Table 1). An alanine to threonine substitution in the second repeat of human Notch3 is associated with CADASIL, a hereditary adult-onset condition causing stroke and dementia (Joutel et al. 1996). This mutation has been mapped onto the structure of highly homologous Drosophila Notch and biophysical characterizations show that the secondary structure and unfolding transition of the mutant protein did not deviate significantly from wild type (Zweifel et al. 2003). These studies strongly suggest that the CADASIL mutation, which is located on the surface of the protein, disrupts specific contacts at the protein–protein interaction interface.
Bare lymphocyte syndrome (BLS) is a disease in which CD4(+) lymphocytes fail to present antigenic peptides to the cellular surface, causing a combined immunodeficiency. Individuals with this disease have defects in at least one of four transcriptional regulatory elements, including the RFXANK gene. RFXANK forms a heterotrimeric complex with RFXAP and RFX5, which can then bind DNA and recruit CIITA to trigger transcription of MHCII genes (Masternak et al. 1998; DeSandro et al. 2000). In the absence of a high-resolution structure for RFXANK, modeling the C-terminal four ankyrin repeats onto the existing crystal structure of GABP- and Swi6, followed by subsequent mutagenesis studies allowed Nekrep et al. (2001) to identify residues participating in the protein–protein interaction interface. Interestingly, mutagenesis studies indicated that RFXAP and CIITA bind to opposite surfaces of RFXANK, and that RFXAP interacts along two different surfaces of the ankyrin repeats. The BLS-associated mutation D121V is located in the -hairpin loop of the first repeat and completely inactivates RFXANK (Wiszniewski et al. 2003). This mutation may replace a contact residue and reduce the affinity for one of the protein–protein interaction partners (Table 1). Mutation L195P is located in the inner helix of the third repeat of RFXANK and presumably disrupts the helix, abolishing the interaction with binding partners RFX5 and RFXAP (Nekrep et al. 2001). Taken together, these three proteins represent testable models of disease-related ankyrin repeat proteins that are amenable to further biophysical and structural characterization.
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Consensus design studies |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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Consensus-based design strategies have been previously applied to a number of proteins with excellent results. The zinc finger was one of the first motifs to be successfully designed using the existing sequences to engineer DNA binding specificity (Desjarlais and Berg 1993). Early design studies on the coiled-coil to understand the mechanisms of oligomerization, and the contribution of various positions to stability incorporated knowledge of available sequences (Harbury et al. 1993; Zhou et al. 1994). Sequence information has also been used to engineer greater thermostability in proteins. Alignment of 13 fungal phytase sequences was used to create a consensus phytase with normal enzymatic activity but significantly higher thermostability (Lehmann et al. 2000). Finally, a 33-residue WW domain consensus sequence was determined from approximately 60 known WW domain sequences and high resolution structural studies showed this "prototype" WW domain to be well-folded (Macias et al. 2000).
Unlike the protein domains previously chosen for consensus design, the ankyrin repeat has a significantly larger number of identified sequences available. In addition, the modular nature provides an excellent "generic" surface that can theoretically interact with any protein. We performed statistical analysis on ~4400 ankyrin repeat sequences downloaded from the PFAM database to create a consensus or idealized ankyrin repeat sequence (Mosavi et al. 2002a). Our analyses consisted of classifying the conservation-level of each position based on the amino acid occurrence at that position, the amino acid distribution over the entire sequence, and the secondary structure elements corresponding to each position (Fig. 3A). We used this sequence to construct proteins consisting of one, two, three, or four identical repeats (1ANK
[PDB]
, 2ANK, 3ANK, and 4ANK respectively). Biophysical characterization showed 3ANK and 4ANK to be fully folded and monomeric, while 2ANK was partially folded and 1ANK
[PDB]
was completely unfolded. Thermal denaturation studies on 3ANK and 4ANK revealed that these designed proteins had higher thermostability than any previously characterized, naturally occurring ankyrin repeat proteins of equivalent size. High-resolution X-ray crystal structures of 3ANK and 4ANK showed well-packed highly regular ankyrin repeat structures with a periodic network of hydrogen bonding (Fig. 2o–p). These results demonstrate that a statistically derived consensus sequence contains all the necessary structural information for the ankyrin repeat fold.
Subsequently, similar work utilizing consensus design on ankyrin repeats with some significant differences was published by Binz et al. They started with statistical analysis of 229 repeats from the SMART database, and included information from 10 existing high-resolution structures of ankyrin repeat proteins to create a consensus ankyrin sequence (Binz et al. 2003). Their strategy incorporated "capping" repeats derived from the structure of GABP- to flank the N and C termini of the protein. In addition, for the internal repeats, they allowed positions in the shorter helix and -hairpin region (2, 3, 5, 13, 14, and 33) to contain any amino acid except glycine, proline, or cysteine. Six randomly selected library members with four, five, or six repeats were characterized biophysically showing well-folded structures with high stability. X-ray crystallography of E3_5 (a five-repeat library member) showed well-packed side chains adopting a regular ankyrin repeat fold (Fig. 2n; Kohl et al. 2003).
Design of "generic" ankyrin repeat proteins is possible using consensus sequence design, as demonstrated by these two independent studies. Despite the variations in design strategy, 3ANK, 4ANK, and E3_5 formed well-folded proteins and displayed higher stability than any previously characterized ankyrin repeat proteins. These results demonstrate that statistical analysis of sequence databases is an effective way to design highly stable protein frameworks that serve as a foundation for more specific protein–protein interactions. As more sequence data become available, additional protein folds can be manipulated using this method.
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Conclusions |
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TOPAbstractIntroductionStructural features of the...Folding and stability of...Ankyrin repeat-mediated protein...Disease-related defects in...Consensus design studiesConclusionsReferences |
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Acknowledgments |
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