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Figure 1. Crystal structures of Ag(I)-bound CusF. (A) Backbone overlay of apo-CusF (blue) and CusF-Ag(I) (green) structure showing the Ag(I)-coordinating residues. (B) Stereo image of the 1.0 Å 2F o–F c electron density map (contoured at 1.5
) of the binding-site region. Phases were calculated using all atoms except those from H36, M47, and M49 and the Ag(I) ion. Residues H36, W44, and both side-chain conformations of M47 and M49 (cyan-minor conformation, green-major conformation) are shown. The figure was generated using PyMOL (DeLano 2002) and Adobe Photoshop.