Solution structure of Ca²⁺-free rat -parvalbumin (oncomodulin)

Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (876 KB) - Supplemental Figure S1: a comparison of the 1H, 15N-HSQC spectra of apo- and Ca2+-bound rat beta-PV.
  Supplemental Figure S2: a depiction of the sequential Csuperscriptalpha and Csuperscriptbeta connectivities between residues 32-41 of Ca2+-free rat beta-PV.
  Supplemental Figure S3: the sequential distribution of NMR restraints used in the calculations.
  Supplemental Figure S4: representative 15N relaxation data (Rsubscript1, Rsubscript2) with corresponding least-squares fits.
  Supplemental Tables: (1) contains the numerical estimates of inter-helical contact in apo- and Ca2+-bound beta-PV; (2) lists the Rsubscript1, Rsubscript2, and NOE values measured for Ca2+-free rat beta-PV; (3) lists the Lipari-Szabo model-free parameters obtained by fitting the Rsubscript1, Rsubscript2 and NOE data.