Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File(343 KB) - Figure S-1. Effect of hydrogen ion concentration on reaction rate. Assays of E. faecalis phosphomevalonate kinase activity were conducted at the indicated pH values under otherwise standard conditions.
  
  Figure S-2. Effect of temperature on reaction rate. Assays of E. faecalis phosphomevalonate kinase activity were conducted at the indicated temperatures under otherwise standard conditions.
  Inset: Selected data are shown as an Arrhenius plot.
  
  Figure S-3. Km for (R,S)-5-phosphomevalonate. Assays of phosphomevalonate kinase activity were conducted at the indicated concentrations of phosphomevalonate under otherwise standard conditions. Specific activity is expressed as micromoles/min/mg.
  
  Figure S-4. Km for ATP. Assays of phosphomevalonate kinase activity were conducted at the indicated concentrations of ATP under otherwise standard conditions. Specific activity is expressed as micromoles/min/mg.
  
  Figure S-5. SDS-PAGE of purified E. faecalis phosphomevalonate kinase. Shown are molecular mass standards (left lane) and fractions 5-12 from the Ni++ column.