Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (1.38 MB) - ESM includes three gels of control reactions for IsoT cleavage assays. These include (1) demonstration of successful IsoT cleavage of the Ub2 constructs containing amino acid residue modifications of the free C-terminus (proximal Ub), a replacement of Gly76 with a cysteine and an extension by Asp77, (2) a native gel showing mobility of the Ub-HBVS-Ub construct (no isopeptide linkage), and (3) a demonstration that a covalent modification of the side chain of Cys76 (proximal Ub) and of both Gly76 (proximal Ub) and Cys48 (distal Ub) hinders the IsoT cleavage of Ub2.