Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (515 KB) - 1) Annotated sequence maps of the pSOS, pSOX, and pSOS-X vectors
  2) A spreadsheet listing the genetic elements of the above vectors
  3) Several tables indicating the outcome of our various optimization efforts
  4) Expanded Materials and Methods covering those optimizations and other routine molecular biology steps
  In addition to the ESM, we host the following components at http://www.biochem.duke.edu/hellinga/pfa.html:
  1) The GeneFab and FabMgr software suite and documentation
  2) The liquid class definitions file we have compiled for use on a Tecan robotic platform running Gemini software for PFA
  3) Tecan-specific scripts and software for PCR machine loading and thermal cycling automation