Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (192 KB) - Suppl. Table 1. Glucose-bound GGBP refinement process.
  Figure S1. The beta-anomer of glucose is bound to GGBP in solution. Overlay of 13C- NMR spectra of the anomeric position of 13C-glucose in the presence (top spectrum) and absence (bottom spectrum) of GGBP. Unbound alpha- and beta-glucose anomeric carbon doublets are visible in the presence of GGBP (top). The broadened doublet of the anomeric position of GGBP-bound beta-glucose is present 0.5 ppm upfield of the unbound doublet. There is no similarly shifted, broadened doublet near the a anomeric carbon doublet, indicating that alpha-glucose is not bound by GGBP .
  Figure S2. Key water molecules for hinge motion. Stereo views of residues G 109-T-Ylll, L255-N-D257, and V293-P-Y295 were chosen to represent hinge segments one, two and three, respectively. (a) The glucose-bound structure contains two structurally important waters. W1 connects segments two and three, while W2 bridges segments one and three. (b) In the 31° opened structure W 1 is also present. However, the water bridge formed by W2 is replaced by direct hydrogen-bonding between segments one and three.