Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase
Protein Sci Puhl et al.
16: 1368
Supplemental Research Data
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Supplemental 1. Time course of PhyAsr inactivation by 10mM iodoacetate (IAA). Residual activity is plotted as a function of time incubated with IAA relative to a control reaction.
Supplemental 2. The effect of varying concentration of Ins Psubscript6 on the inactivation of PhyAsr by IAA. The modification reaction was repeated in the presence of 1, 2, 4, and 6 mM sodium phytate. Average activity of the protected and unprotected, modified enzyme relative to an un-alkylated control for three separate experiments is presented.