Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (725 KB) - Supplementary Figure 1: A typical SPR sensorgram for tensin1 binding to immobilized integrin tails obtained at six different concentrations of tensin1 (1.61 microM-50 microM), from which Kd values in Table 2 were obtained.
  Supplementary Figure 2: SPR sensorgrams of wild-type and mutant tensin1 PTB domains binding to integrin tail. Experiments were carried using a single fixed concentration (10 microM) of PTB domain injected for 1 minute at 20 microl/min over a CM5 chip immobilized with non-phosphorylated integrin beta1A (residues 779-790).
  Supplementary Figure 3: Differential Scanning Calorimetry of wild-type and mutant tensin1 PTB domains. With the exception of F1682A, the similar melting profiles are similar to wild-type, indicating well-folded proteins, while the moderate reduction in Tm suggests a small decrease in rigidity.