Supplemental Research Data

Files in this Data Supplement:

• Adobe PDF File (1.4MB) - Supplementary Figure 1. Expression and purification of wild type cC273 and cC273 with one or both of the N-terminal or C-terminal extensions removed. All proteins were expressed and purified under identical conditions. The picture of the SDS PAGE separation shows the whole cell lysate, supernatant, and pellet after sonification for wild type and truncated cC273. In each section, lane 1 is wild type cC273, lane 2 is cC273 with the C-terminal extension deleted, lane 3 is cC273 with both extensions deleted, and lane 4 is cC273 with the N-terminal extension deleted. Asterisks denote expected positions of each protein on the gel. The Superdex-200 (S200) gel filtration profiles of wild type and truncated C273 proteins are shown.
  Supplementary Figure 2. Multiple sequence alignment of representative MPN proteins constructed using MultAlin (Corpet 1988). Prefixes h, c, and y represent human, C. elegans, and yeast proteins, respectively. cPrp8 refers to cC273. Residues highlighted in blue and red are over 50% and 90% conserved in these proteins, respectively. Arrows labeled “N-MPN” and “C-MPN” denote the N- and C-termini of the MPN domain.
  Supplementary Figure 3. Superimposition of cC273 (cyan) and scPrp8Psuperscript2147-2393 (yellow). Labels 1-3 are examples loops that have different conformations between the two structures.