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Specificity and reactive loop length requirements for crmA inhibition of serine proteases

¹ Center for Molecular Biology of Oral Diseases, College of Dentistry and ² Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA

(RECEIVED September 7, 2004; FINAL REVISION October 7, 2004; ACCEPTED October 8, 2004)

The viral serpin, crmA, is distinguished by its small size and ability to inhibit both serine and cysteine proteases utilizing a reactive loop shorter than most other serpins. Here, we characterize the mechanism of crmA inhibition of serine proteases and probe the reactive loop length requirements for inhibition with two crmA reactive loop variants. P1 Arg crmA inhibited the trypsin-like proteases, thrombin, and factor Xa, with moderate efficiencies (~10²–10⁴ M^–1sec^–1), near equimolar inhibition stoichiometries, and formation of SDS-stable complexes which were resistant to dissociation (k_diss ~10^–7 sec^–1), consistent with a serpin-type inhibition mechanism. Trypsin was not inhibited, but efficiently cleaved the variant crmA as a substrate (k_cat/K_M of ~10⁶ M^–1 sec^–1). N-terminal sequencing confirmed that the P1 Arg–P1'Cys bond was the site of cleavage. Altering the placement of the Arg in a double mutant P1 Gly-P1'Arg crmA resulted in minimal ability to inhibit any of the trypsin family proteases. This variant was cleaved by the proteases ~10-fold less efficiently than P1 Arg crmA. Surprisingly, pancreatic elastase was rapidly inhibited by wild-type and P1 Arg crmAs (10⁵–10⁶ M^–1sec^–1), although with elevated inhibition stoichiometries and higher rates of complex dissociation. N-terminal sequencing showed that elastase attacked the P1'Cys–P2'Ala bond, indicating that crmA can inhibit proteases using a reactive loop length similar to that used by other serpins, but with variations in this inhibition arising from different effective P2 residues. These results indicate that crmA inhibits serine proteases by the established serpin conformational trapping mechanism, but is unusual in inhibiting through either of two adjacent reactive sites.

Keywords: serpin; protease; crmA; protease inhibitor; serine protease; mutagenesis

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.041104905.

Reprint requests to: Steven T. Olson, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, Room 530E Dentistry (M/C 860), 801 S. Paulina Street, Chicago, IL 60612, USA; e-mail: stolson{at}uic.edu; fax: (312) 413-1604.

³ These authors contributed equally to this work.

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