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PROTEIN STRUCTURE REPORT

NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependant phosphotransferase system and its interaction with GatA

¹ Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
² Macromolecular Structure Group, Biotechnology Research Institute, Montreal, Quebec H4P 2R2, Canada

(RECEIVED May 10, 2006; FINAL REVISION June 29, 2006; ACCEPTED June 30, 2006)

The phosphoenolpyruvate-dependant carbohydrate transport system (PTS) couples uptake with phosphorylation of a variety of carbohydrates in prokaryotes. In this multienzyme complex, the enzyme II (EII), a carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five families of EIIs identified in Escherichia coli, the galactitol-specific transporter (II^gat) belongs to the glucitol family and is structurally the least well-characterized. Here, we used nuclear magnetic resonance (NMR) spectroscopy to solve the three-dimensional structure of the IIB subunit (GatB). GatB consists of a central four-stranded parallel -sheet flanked by -helices on both sides; the active site cysteine of GatB is located at the beginning of an unstructured loop between 1 and 1 that folds into a P-loop-like structure. This structural arrangement shows similarities with other IIB subunits but also with mammalian low molecular weight protein tyrosine phosphatases (LMW PTPase) and arsenate reductase (ArsC). An NMR titration was performed to identify the GatA-interacting residues.

Keywords: phosphotransferase system; GatB; structure determination; NMR; galactitol

Supplemental material: see www.proteinscience.org

Reprint requests to: Laurent Volpon, Institut de Recherche en Immunologie et Cancérologie, Université de Montréal, Pavillon Marcelle-Coutu, 2950, Chemin Polytechnique, Montreal, Quebec H3T 1J4, Canada; e-mail: laurent.volpon{at}umontreal.ca; fax: (514) 343-5839.

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.062337406.

Abbreviations: GatA, IIA domain of the galactitol specific transporter; GatB, IIB domain of the galactitol specific transporter; PTS, phosphotransferase system; EI, enzyme I; HPr, histidine-containing phosphocarrier protein; EII, enzyme II; LMW PTPase, low molecular weight protein tyrosine phosphatase; PEP, phosphoenolpyruvate; TOCSY, total correlation spectroscopy; NOESY, nuclear Overhauser effect spectroscopy; HSQC, heteronuclear single quantum correlation; hNOE, heteronuclear nuclear Overhauser effect; gat, galactitol; cel, cellobiose; glc, glucose; mtl, mannitol; lac, lactose; man, mannose.

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