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Published online before print May 1, 2007
Protein Science, DOI: 10.1110/ps.072798707
 Copyright © 2007 The Protein Society
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PROTEIN STRUCTURE REPORT

Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions

Clare J. McCleverty1,3, Diane C. Lin2, and Robert C. Liddington1

1 Cancer Center, Burnham Institute for Medical Research, La Jolla, California 92037, USA
2 Department of Developmental and Cell Biology, University of California, Irvine, California 92697, USA

(RECEIVED January 29, 2007; FINAL REVISION March 1, 2007; ACCEPTED March 1, 2007)

Tensin is a cytoskeletal protein that links integrins to the actin cytoskeleton at sites of cell-matrix adhesion. Here we describe the crystal structure of the phosphotyrosine-binding (PTB) domain of tensin1, and show that it binds integrins in an NPxY-dependent fashion. Alanine mutagenesis of both the PTB domain and integrin tails supports a model of integrin binding similar to that of the PTB-like domain of talin. However, we also show that phosphorylation of the NPxY tyrosine, which disrupts talin binding, has a negligible effect on tensin binding. This suggests that tyrosine phosphorylation of integrin, which occurs during the maturation of focal adhesions, could act as a switch to promote the migration of tensin–integrin complexes into fibronectin-mediated fibrillar adhesions.

Keywords: structure/function studies; crystallography; calorimetry; mutagenesis (site-directed and general); docking proteins; surface plasmon resonance

3 Present address: Protein Sciences, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr., San Diego, CA 92121, USA.

Supplemental material: see www.proteinscience.org

Reprint requests to: Robert C. Liddington, Cancer Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA; e-mail: rlidding{at}burnham.org; fax: (858) 713-9925.

Abbreviations: PTB, phosphotyrosine-binding (domain); ECM, extracellular matrix; SH2, Src homology 2; PIPKI{gamma}, phosphatidylinositol phosphate kinase type I{gamma} ; EDC, N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride; NHS, N-hydroxysuccinimide; RMSD, root mean square deviation; SPR, surface plasmon resonance; DSC, differential scanning calorimetry.

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.072798707.


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