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Functional analysis of the GTPases EngA and YhbZ encoded by Salmonella typhimurium

¹ Institute of Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
² Wolfson Institute for Biomedical Research, University College London, London WC1E 6AU, United Kingdom
³ Arrow Therapeutics, Britannia House, London SE1 1DA, United Kingdom
⁴ Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, Untied Kingdom
⁵ Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom
⁶ Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, United Kingdom

(RECEIVED March 26, 2007; FINAL REVISION July 10, 2007; ACCEPTED August 20, 2007)

The S. typhimurium genome encodes proteins, designated EngA and YhbZ, which have a high sequence identity with the GTPases EngA/Der and ObgE/CgtA_E of Escherichia coli. The wild-type activity of the E. coli proteins is essential for normal ribosome maturation and cell viability. In order to characterize the potential involvement of the Salmonella typhimurium EngA and YhbZ proteins in ribosome biology, we used high stringency affinity chromatography experiments to identify strongly binding ribosomal partner proteins. A combination of biochemical and microcalorimetric analysis was then used to characterize these protein:protein interactions and quantify nucleotide binding affinities. These experiments show that YhbZ specifically interacts with the pseudouridine synthase RluD (K_D = 2 µM and 1:1 stoichiometry), and we show for the first time that EngA can interact with the ribosomal structural protein S7. Thermodynamic analysis shows both EngA and YhbZ bind GDP with a higher affinity than GTP (20-fold difference for EngA and 3.8-fold for YhbZ), and that the two nucleotide binding sites in EngA show a 5.3-fold difference in affinity for GDP. We report a fluorescence assay for nucleotide binding to EngA and YhbZ, which is suitable for identifying inhibitors specific for this ligand-binding site, which would potentially inhibit their biological functions. The interactions of YhbZ with ribosome structural proteins that we identify may demonstrate a previously unreported additional function for this class of GTPase: that of ensuring delivery of rRNA modifying enzymes to the appropriate region of the ribosome.

Keywords: EngA; YhbZ; RluD; microcalorimetry; ribosome

Reprint requests to: Alastair R. Hawkins, Institute of Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK; e-mail a.r.hawkins{at}ncl.ac.uk; fax +44 (0)-191-2227673.

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.072900907.

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