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FOR THE RECORD

Contribution of dipole–dipole interactions to the stability of the collagen triple helix

Istituto di Biostrutture e Bioimmagini, CNR, I-80134 Napoli, Italy

(RECEIVED October 18, 2007; FINAL REVISION December 19, 2007; ACCEPTED December 19, 2007)

Unveiling sequence–stability and structure–stability relationships is a major goal of protein chemistry and structural biology. Despite the enormous efforts devoted, answers to these issues remain elusive. In principle, collagen represents an ideal system for such investigations due to its simplified sequence and regular structure. However, the definition of the molecular basis of collagen triple helix stability has hitherto proved to be a difficult task. Particularly puzzling is the decoding of the mechanism of triple helix stabilization/destabilization induced by imino acids. Although the propensity-based model, which correlates the propensities of the individual imino acids with the structural requirements of the triple helix, is able to explicate most of the experimental data, it is unable to predict the rather high stability of peptides embedding Gly–Hyp–Hyp triplets. Starting from the available X-ray structures of this polypeptide, we carried out an extensive quantum chemistry analysis of the mutual interactions established by hydroxyproline residues located at the X and Y positions of the Gly–X–Y motif. Our data clearly indicate that the opposing rings of these residues establish significant van der Waals and dipole–dipole interactions that play an important role in triple helix stabilization. These findings suggest that triple helix stabilization can be achieved by distinct structural mechanisms. The interplay of these subtle but recurrent effects dictates the overall stability of this widespread structural motif.

Keywords: collagen; triple helix; protein–protein association; imino acids; quantum chemistry

Reprint requests to: Roberto Improta, Istituto di Biostrutture e Bioimmagini, Via Mezzocannone 16, I-80138 Napoli, Italy; e-mail: robimp{at}unina.it; fax: 39-81-674190; or Rita Berisio, Istituto di Biostrutture e Bioimmagini, Via Mezzocannone 16, I-80138 Napoli, Italy; e-mail: rita.berisio{at}unina.it; fax: 39-81-674190.

Abbreviations: Hyp, (4R,2S)-hydroxyproline; hyp, (4S,2S)-hydroxyproline; Flp, (4R,2S)-fluoroproline; flp, (4S,2S)-fluoroproline; Hyp(X), Pro(X), hydroxyproline, and proline located at the X position; Hyp(Y), Pro(Y), hydroxyproline, and proline located at the Y position.

Article published online ahead of print. Article and publication date are at http://www.proteinscience.org/cgi/doi/10.1110/ps.073301908.

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