Journal Issue - Volume 11 Issue 12 (December 2002)
Mechanically unfolding proteins: The effect of unfolding history and the supramolecular scaffold
- Rebecca C. Zinober, David J. Brockwell, Godfrey S. Beddard, Anthony W. Blake, Peter D. Olmsted, Sheena E. Radford, D. Alastair Smith
- Published in Wiley Interscience on Jan 01, 2009
- DOI: 10.1110/ps.0224602 (p 2759-2765)
Abstract The mechanical resistance of a folded domain in a polyprotein of five mutant I27 domains (C47S, C63S I27)5is shown to depend on the unfolding history of the protein. This observation can be understood on the basis of competition between two effects, that of the changing number of domains attempting to unfold, and the progressive increase in the compliance of the polyprotein as domains unfold. We present Monte Carlo simulations that...
The interaction of β 2 ‐glycoprotein I domain V with chaperonin GroEL: The similarity with the domain V and membrane interaction
- Masayo Gozu, Masaru Hoshino, Takashi Higurashi, Hisao Kato, Yuji Goto
- Published in Wiley Interscience on Jan 01, 2009
- DOI: 10.1110/ps.0216602 (p 2792-2803)
Abstract To clarify the mechanism of interaction between chaperonin GroEL and substrate proteins, we studied the conformational changes; of the fifth domain of human β2‐glycoprotein I upon binding to GroEL. The fifth domain has a large flexible loop, containing several hydrophobic residues surrounded by positively charged residues, which has been proposed to be responsible for the binding of β2‐glycoprotein I to negatively charged phospholipid...
Partly folded states of members of the lysozyme/lactalbumin superfamily: A comparative study by circular dichroism spectroscopy and limited proteolysis
- Patrizia Polverino de Laureto, Erica Frare, Rossella Gottardo, Herman van Dael, Angelo Fontana
- Published in Wiley Interscience on Jan 01, 2009
- DOI: 10.1110/ps.0205802 (p 2932-2946)
Abstract The partly folded states of protein members of the lysozyme (LYS)/α‐lactalbumin (LA) superfamily have been analyzed by circular dichroism (CD) measurements and limited proteolysis experiments. Hen, horse, dog, and pigeon LYSs and bovine LA were used in the present study. These are related proteins of 123‐ to 129‐amino‐acid residues with similar three‐dimensional structures but low similarity in amino acid sequences....
