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Journal Issue - Volume 2 Issue 1 (January 1993)

  • First anniversary

  • Hans Neurath
  • Published in Wiley Interscience on Dec 31, 2008
  • DOI: 10.1002/pro.5560020101 (p 1-1)

Abstract The nucleic acid interactive properties of a synthetic peptide with sequence of the N‐terminal CCHC zinc finger (CCHC = Cys‐X2‐Cys‐X4‐His‐X4‐Cys; × = variable amino acid) of the human immunodeficiency virus (HIV) nucleocapsid protein, Zn(HIV1‐F1), have been studied by 1H NMR spectroscopy. Titration of Zn(HIV1‐F1) with oligodeoxyribonucleic acids containing different nucleotide sequences reveals, for the first time, sequence‐dependent...

Abstract We demonstrate that three integral membrane receptors of mammals — the ecotropic retroviral leukemia receptor (ERR), the human retroviral receptor (HRR), and the T‐cell early activator (Tea) — are homologous to a family of transporters specific for amino acids, polyamines, and choline (APC), which catalyze solute uniport, solute:cation symport, or solute:solute antiport in yeast, fungi, and eubacteria. Interestingly, the...

Abstract Kinases that catalyze phosphorylation of sugars, called here sugar kinases, can be divided into at least three distinct nonhomologous families. The first is the hexokinase family, which contains many prokaryotic and eukaryotic sugar kinases with diverse specificities, including a new member, rhamnokinase from Salmonella typhimurium. The three‐dimensional structure of hexokinase is known and can be used to build models of functionally...

Abstract This paper examines the topological properties of protein disulfide bonding patterns. First, a description of these patterns in terms of partially directed graphs is developed. The topologically distinct disulfide bonding patterns available to a polypeptide chain containing n disulfide bonds are enumerated, and their symmetry and reducibility properties are examined. The theoretical probabilities are calculated that a...

Abstract Amino acid substitution tables are calculated for residues in membrane proteins where the side chain is accessible to the lipid. The analysis is based upon the knowledge of the three‐dimensional structures of two homologous bacterial photosynthetic reaction centers and alignments of their sequences with the sequences of related proteins. The patterns of residue substitutions show that the lipid‐accessible residues are less...

Abstract The zinc metalloenzyme porphobilinogen synthase (PBGS) contains several functionally important, but previously unidentified, reactive sulfhydryl groups. The enzyme has been modified with the reversible sulfhydryl‐specific nitroxide spin label derivative of methyl methanethiosulfonate (MMTS), (1‐oxyl‐2,2,5,5‐tetramethyl‐Δ3‐pyrroline‐3‐methyl)methanethiosulfonate (SL‐MMTS) (Berliner, L.J., Grunwald, J., Hankovszky, H.O., & Hideg, K.,...

Abstract The helix‐stabilizing effects of repeating pairs of Asp‐Arg and Glu‐Arg residues have been characterized using a peptide system of the same design used earlier to study Glu‐Lys (Marqusee, S. & Baldwin, R.L., 1987, Proc. Natl. Acad. Sci. USA 84, 8898–8902) and Asp‐Lys ion pairs (Marqusee, S. & Baldwin, R.L., 1990, In Protein Folding [Gierasch, L.M. & King, J., Eds.], pp. 85–94, AAAS, Washington, D.C.). The consequences of breaking ion...

Abstract Four tyrosine residues have been identified as phosphorylation sites in the tyrosine kinase isoform of the heparin‐binding fibroblast growth factor receptor flg (FGF‐R1). Baculoviral‐insect cell‐derived recombinant FGF‐R1 was phosphorylated and fragmented with trypsin while immobilized on heparin‐agarose beads. Phosphotyrosine peptides were purified by chromatography on immobilized anti‐phosphotyrosine antibody and analyzed...

Abstract MgATP‐binding and hydrolysis are an integral part of the nitrogenase catalytic mechanism. We are exploring the function of MgATP hydrolysis in this reaction by analyzing the properties of the Fe protein (FeP) component of Azotobacter vinelandii nitrogenase altered by site‐directed mutagenesis. We have previously (Seefeldt, L.C., Morgan, T.V., Dean, D.R., & Mortenson, L.E., 1992, J. Biol. Chem. 267, 6680–6688) identified a region...

Abstract Interaction of a 70‐amino acid zinc‐binding polypeptide from the regulatory chain of aspartate transcarbamoylase (ATCase) with the catalytic (C) subunit leads to dramatic changes in enzyme activity and affinity for ligand binding at the active sites. The complex between the polypeptide (zinc domain) and wild‐type C trimer exhibits hyperbolic kinetics in contrast to the sigmoidal kinetics observed with the intact holoenzyme....

Abstract This work investigates the influence of storage of lysozyme in solution on its crystallization. The crystallization of hen egg‐white lysozyme exhibits a storage effect (aging) that depends on the length of time the lysozyme solution is stored, after dissolving from freeze‐dried powder, before being brought to crystallization conditions. The number of crystals obtained increases, while their size decreases, as the solution...

Abstract Potato tuber UDP‐glucose pyrophosphorylase (EC 2.7.7.9) catalyzes the reversible uridylyl transfer from UDP‐glucose to MgPPi forming glucose 1‐phosphate and MgUTP, according to an ordered bi‐bi mechanism in which UDP‐glucose and MgPPi bind in this order. To probe the active site of this enzyme, we have applied pyridoxal 5′‐diphosphate, a reactive PPi analogue. The enzyme was rapidly inactivated when incubated with the reagent in the presence of Mg2+ followed...

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