Heat capacity change for ribonuclease A folding

The change in heat capacity ΔC_p for the folding of ribonuclease A was determined using differential scanning calorimetry and thermal denaturation curves. The methods gave equivalent results, ΔC_p = 1.15 ± 0.08 kcal mol^–1 K^–1. Estimates of the conformational stability of ribonuclease A based on these results from thermal unfolding are in good agreement with estimates from urea unfolding analyzed using the linear extrapolation method.