Article
Received: 3 December 2008; Revised: 16 February 2009; Accepted: 17 February 2009
10.1002/pro.106 About DOI
Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks |
| Kengo Usui 1 2 3, Tei Maki 1 4, Fuyu Ito 1 2 3, Atsushi Suenaga 5, Satoru Kidoaki 4, Masayoshi Itoh 2 3, Makoto Taiji 5, Takehisa Matsuda 6, Yoshihide Hayashizaki 2 3, Harukazu Suzuki 2 3 * |
| 1CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan 2Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), Tsurumi-ku, Yokohama 230-0045, Japan 3Genome Science Laboratory, RIKEN, Hirosawa, Wako 351-0198, Japan 4Division of Biomolecular Chemistry, Kyushu University, Fukuoka 812-8581, Japan 5Computational and Experimental System Biology Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230-0046, Japan 6Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Ishikawa 924-0838, Japan |
| email: Harukazu Suzuki (rgscerg@gsc.riken.jp) |
*Correspondence to Harukazu Suzuki, Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
Funded by:
CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation RIKEN Genome Exploration Research Project, MEXT of Japanese Government
| Keywords |
| self-assembly nanofilament protein design molecular modeling protein nanomaterial |
| Abstract |
Self-assembly of artificially designed proteins is extremely desirable for nanomaterials. Here we show a novel strategy for the creation of self-assembling proteins, named  Nanolego. Nanolego consists of structural elements of a structurally stable symmetrical homo-oligomeric protein and binding elements, which are multiple heterointeraction proteins with relatively weak affinity. We have established two key technologies for Nanolego, a stabilization method and a method for terminating the self-assembly process. The stabilization method is mediated by disulfide bonds between Cysteine-residues incorporated into the binding elements, and the termination method uses capping Nanolegos, in which some of the binding elements in the Nanolego are absent for the self-assembled ends. With these technologies, we successfully constructed timing-controlled and size-regulated filament-shape complexes via Nanolego self-assembly. The Nanolego concept and these technologies should pave the way for regulated nanoarchitecture using designed proteins. |
Received: 3 December 2008; Revised: 16 February 2009; Accepted: 17 February 2009
| Digital Object Identifier (DOI) |
10.1002/pro.106 About DOI
