ArticleCyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin |
| Wansong Zong, Rutao Liu *, Feng Sun, Meijie Wang, Pengjun Zhang, Yihong Liu, Yanmin Tian |
School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China
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| email: Rutao Liu (rutaoliu@sdu.edu.cn) |
*Correspondence to Rutao Liu, School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China
Funded by:
NSFC; Grant Number: 20607011, 20875055
NCET-06-0582,
the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China; Grant Number: 708058
Excellent Young Scientists and Key Science-Technology Project in Shandong Province; Grant Number: 2007BS08005, 2008GG10006012
| bovine insulin oxidative damage evaluation strategy cyclic voltammetry |
Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond S SCYS7A,CYS7B, forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO2H and sulfonic acid RSO3H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin. |
Received: 26 August 2009; Revised: 7 November 2009; Accepted: 27 November 2009
10.1002/pro.313
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